Articles

Isolation ofanewbutanol-producing Clostridium strain: Highlevel of hemicellulosicactivityandstructureofsolventogenesisgenes of anew Clostridium saccharobutylicum isolate

Oksana V.Berezinaa,, AgnieszkaBrandtb, SergeyYarotskya, Wolfgang H.Schwarzb, VladimirV.Zverlovb, 

cState ResearchInstituteofGeneticsandSelectionofIndustrialMicroorganisms,1stDorojniypr.1,117545Moscow, Russian Federation

bDepartment ofMicrobiology,TechnischeUniversita¨t Mu¨nchen, AmHochanger4,85350Freising,Germany 

cInstitute ofMolecularGenetics,RussianAcademyofScience,KurchatovSq.2,123182Moscow,RussianFederation

 

Abstract

New isolates of solventogenic bacteria exhibitedhighhemicellulolyticactivity.Theyproducedbutanoland acetone withhighselectivityforbutanol(about80%ofbutanolfromthetotalsolventyield).Their16SrDNA sequencewas99%identicaltothatof Clostridiumsaccharobutylicum. Thegenesresponsibleforthelaststeps of solventogenesisandencodingcrotonase,butyryl-CoAdehydrogenase,electron-transportproteinsubunitsAandB, 3-hydroxybutyryl-oAdehydrogenase,alcoholdehydrogenase,CoA-transferase(subunitsAandB),acetoacetate decarboxylase,andaldehydedehydrogenasewereidentifiedinthenew C. saccharobutylicum strainOx29andcloned into Escherichiacoli. The genesforcrotonase,butyryl-CoAdehydrogenase,electron-transportproteinsubunitsA and B,and3-hydroxybutyryl-CoAdehydrogenasecomposedthe bcs-operon.Amonocistronicoperoncontaining the alcoholdehydrogenase gene was located downstream of the bcs-operon.Genesforaldehydedehydrogenase, CoA-transferase(subunitsAandB),andacetoacetatedecarboxylasecomposedthe sol-operon. The gene sequences  and thegeneorderwithinthe sol- and bcs-operons of C. saccharobutylicum Ox29 were most similar to those of Clostridiumbeijerinckii. The activity of some of the bcs-operon genes,expressed in heterologous E. coli, was determined. 

A novel model system for design of biomaterials based on recombinant analogs of spider silk proteins

Bogush VG, Sokolova OS, Davydova LI, Klinov DV, Sidoruk KV, Esipova NG, Neretina TV, Orchanskyi IA, Makeev VY, Tumanyan VG, Shaitan KV, Debabov VG, Kirpichnikov MP.
Neuroimmune Pharmacol. 2009 Mar;4(1):17-27. Epub 2008 Oct 7.

Screening, Characterization and Application of Cyanide-resistant Nitrile Hydratases

The Threonine Story

Vladimir G. Debabov

Advances in biochemical engineering/biotechnology 79:113-36, 2003

 

Nitrile Hydratase of Rhodococcus. Optimization of Synthesis in Cells and Industrial Applications for Acrylamide Production

TATYANA E. LEONOVA (POGORELOVA), OLGA B. ASTAUROVA, LUDMILA E. RYABCHENKO, AND ALEXANDER S. YANENKO*