Genetika Articles Titanium dioxide (TiO2) nanoparticles induce bacterial stress response detectable by specific lux biosensors

Titanium dioxide (TiO2) nanoparticles induce bacterial stress response detectable by specific lux biosensors

Titanium dioxide (TiO2) nanoparticles induce bacterial stress response detectable by specific lux biosensors

G. B. Zavilgelsky, V. Yu. Kotova, I. V. Manukhov

ABSTRACT: To investigate the mechanism of the bactericidal effect of TiO2 nanoparticles, we used induced lux biosensors on the basis of the Escherichia coli MG1655 strain containing pColD-lux, pKatG-lux, and pSoxS-lux plasmids. It was shown that the UV-light (λ = 360–390 nm) treatment of a suspension of biosensor cells and TiO2 nanoparticles induces H2O2 formation in bacteria, which induces the expression of the luxCDABE reporter genes, leading to bioluminescence intensification. It is proposed to use the specifically induced lux biosensors for the detection of photoactive dioxide metal nanoparticles.

Nanotechnologies in Russia 04/2012; 6(5):401-406.

07.04.2012, 2118 просмотров.