Multifunctional regulatory mutation in Bacillus subtilis flavinogenesis system

Multifunctional regulatory mutation in Bacillus subtilis flavinogenesis system

R. A. Kreneva, D. V. Karelov, N. V. Korolkova, A. S. Mironov, D. A. Perumov

ABSTRACT: Among Bacillus subtilis riboflavin-resistant mutants we identified one, which differed from other regulatory mutants by overproduction of riboflavin and simultaneous upregulation of the ribC gene encoding flavokinase/FAD-synthase. Genetic and biochemical analysis showed that the ribU1 mutation determines a trans-acting factor that simultaneously regulates activity of riboflavin and truB-ribC-rpsO operons. Regulatory activity of the ribU1 mutation comprises about 10% of Rfn element activity on interaction with flavins. The ribU1 mutation can be presumably ascribed to a gene of the transcriptional regulators family.

Russian Journal of Genetics 04/2012; 45(10):1256-1259.

07.03.2013, 2987 просмотров.