Uridine Phosphorylase (UDP) from Shewanella oneidensis MR-1: Heterological Expression, Regulation, Transformation and Properties

Автор: N.N. Mordkovich, V.A. Manuvera, V.P. Veiko, and V.G. Debabov

Страница: 21-30

Uridine Phosphorylase (UDP) from Shewanella oneidensis MR-1: Heterological Expression, Regulation, Transformation and Properties

Biotekhnologiya, 2012, N 1, P. 21-30

UDC 577.152.242*3.03:577.112017

Section:  “Producers, Biology, Selection, and Gene Engineering”

N.N. Mordkovich ^*,  V.A. Manuvera,  V.P. Veiko,  and  V.G. Debabov

The State Research Institute for Genetics and Selection of Industrial Microorganisms,  117545, Moscow Russia

e-mail:  lab19@genetika.ru

The fragment containing promoter-operator and structural parts of a gene for uridine phosphorylase from Shewanella oneidensis MR-1 has been cloned. A cross-heterological expression of the udp genes from Sh. oneidensis MR-1 and Escherichia coli under the control of the authentic regulatory regions was shown. The UDP protein was accumulated in the active form in the cytoplasmic fraction. The recombinant UDP protein from Sh. oneidensis MR-1 obtained by the heterological expression was isolated and characterized. The activity of the promoter of the E. coli udp gene upon the heterological expression in Sh. oneidensis MR-1 cells under either aerobic or anaerobic conditions was observed.

Key words:  E. coli,  heterological expression,  Shewanella oneidensis,  uridine phosphorylase (UDP).

The full English version of the article was published in “Applied Biochemistry and Microbiology”, 2012, Vol. 48, Issue 9, pp. 716-722 as N. N. Mordkovich, V. A. Manuvera, V. P. Veiko, V. G. Debabov “Uridine phosphorylase from Shewanella oneidensis MR-1: Heterological expression, regulation, transcription, and properties”.

It is contained at the SpringerLink website:  http://link.springer.com/article/10.1134/S0003683812090037  and  at the Russian Scientific Electron Library website:  http://elibrary.ru/item.asp?id=20490455

DOI: 10.1134/S0003683812090037

15.06.2015, 1642 просмотра.