In planta Agrobacterium-Mediated Plant Transformation Technologies

Автор: M.I. Chumakov, and Yе.М. Moiseeva

Страница: 8-20

in planta Agrobacterium-mediated plant transformation technologies

Biotekhnologiya, 2012, N 1, P. 8-20

UDC 581.1 + 575.224.4

Section:  “Problems and Prospects”

M.I. Chumakov ^{1, 2, *},  and  Yе.М. Moiseeva ¹

¹  The Institute of Biochemistry and Physiology of Plants and Microorganisms, Russ. Acad. Sci.,  410049, Saratov Russia

²  The Saratov State University,  410012, Saratov Russia

e-mail:  chumakov@ibppm.sgu.ru

This review describes methods for the Agrobacterium-mediated T-DNA transfer to plant cells under in planta conditions. The main attention is focused on the techniques for gene transfer with Agrobacterium T-DNA to plant generative cells. The  effects of plant genotype, bacterial strain, type of vector constructs, composition of inoculation medium and conditions for plant treatment with agrobacteria on the efficiency of Agrobacterium-mediated transformation in planta have been analyzed. On the basis of the reported and authors’ own  experimental data, a putative mechanism of the plant generative cells Agrobacterium-mediated transformation in planta is discussed.

Key words:  Agrobacterium tumefaciens,  genetic transformation in planta,  transgenic plants.

The full English version of the article was published in “Applied Biochemistry and Microbiology”, 2012, Vol. 48, Issue 8, pp. 657-666 as M. I. Chumakov, E. M. Moiseeva “Technologies of Agrobacterium plant transformation In planta”.

It is contained at the SpringerLink website:  http://link.springer.com/article/10.1134/S0003683812080017  and  at the Russian Scientific Electron Library website:  http://elibrary.ru/item.asp?id=20489815

DOI: 10.1134/S0003683812080017

15.06.2015, 1902 просмотра.