Studies on Physicochemical Properties of a New Preparation of Deltaferon as a Part of a Molecular Construct

Автор: O.S. Korovina, D.V. Korneev, G.M. Levagina, M.P. Bogryantseva, and V.I. Masycheva

Страница: 73-77

Studies on Physicochemical Properties of a New Preparation of Deltaferon as a Part of a Molecular Construct

Biotekhnologiya, 2012, N 2, P. 73-77

UDC 615.2/3.012:578.245

Section:  “Utilization of Biopreparations”

O.S. Korovina ^*,  D.V. Korneev,  G.M. Levagina,  M.P. Bogryantseva,  and  V.I. Masycheva

The Institute for Medical Biotechnology, State Research Center for virology and Biotechnology Vector,  633010, Berdsk, Novosibirskaya oblast Russia

e-mail:  ol-1986@mail.ru

A molecular construct of the spherical shape containing double-stranded RNA (dsRNA) in the center, coated by spermidine-poliglukin cover and carrying recombinant deltaferon, an analog of recombinant interferon-gamma, has been designed. It was shown that deltaferon retains its specific activity on the level of 9·10⁴ U/mg when included in the above construct. It was shown that deltaferon and dsRNA in the construct were characterized by the enhanced resistance to proteases (trypsin) and nucleases (E. coli ribonuclease) as compared to deltaferon and dsRNA in the free forms. The increased tolerance of deltaferon as a part of the construct to temperature environmental factors during storage was also demonstrated.

Key words:  double-stranded RNA,  interferon-gamma analog of deltaferon,  molecular construct,  resistance to enzymes,  temperature resistance.

The full English version of the article was published in “Applied Biochemistry and Microbiology”, 2013, Vol. 49, Issue 7, pp. 660-663 as O. S. Korovina, D. V. Korneev, G. M. Levagina, M. P. Bogryantseva, V. I. Masycheva “Studies on the physicochemical properties of the new preparation deltaferon as a component of a molecular construct”.

It is contained at the SpringerLink website:  http://link.springer.com/article/10.1134/S0003683813070041  and  at the Russian Scientific Electron Library website:  http://elibrary.ru/item.asp?id=21883450

DOI: 10.1134/S0003683813070041

15.06.2015, 1927 просмотров.