Creation of Oil Rape (Brassica napus) Haploid Plants using Microspore Culture

Автор: T.N. Gribova, A.N. Kniazev, and A.M. Kamionskaya

Страница: 59-65

Creation of Oil Rape (Brassica napus) Haploid Plants using Microspore Culture

Biotekhnologiya, 2012, N 2, P. 59-65

UDC 57.085.23, 58.085

Section:  “Biologicals Technology”

T.N. Gribova ^{1, *}, A.N. Kniazev ²,  and  A.M. Kamionskaya ¹

¹  The Centre for Bioengineering, Russ. Acad. Sci.,  117312, Moscow Russia

²  The Russian State Agrarian University, Timiriazev Moscow Academy of Agriculture,  127550, Moscow Russia

e-mail:  tgribova@biengi.ac.ru

The influence of heat-shock conditions (temperature and duration of exposure) and size of buds on the emryogenesis in a rape microspores culture has been investigated. It was shown, that size of buds has no effect on the formation of haploid plants because embryogenesis is a function of stage of pollen development which is in turn determined by growth condition for the original material. The optimal heat-shock protocol proved to be as follows: for Topaz cultivar, 35°С and 72 h, and for Centr 1 line, 35°С and 24 h. We have obtained 145 embryos, 50 flowering plants of Topaz cultivar, 36 of which turned to be haploid (haploidy frequency of 0,72) and 53 embryos, 16 flowering plants and 4 haploid plants of Centr 1 line (haploidy frequency of 0,25). The haploid nature of plants was proved by the cytological analyses.

Key words:  Brassica napus,  double haploids,  haploids,  microspore culture,  oil rape.

The full English version of the article was published in “Biotechnology in Russia”, 2012, Issue 2, pp. 59-65 as T.N. Gribova, A.N. Kniazev, A.M. Kamionskaya “Creation of Oil Rape (Brassica napus) Haploid Plants using Microspore Culture”.

It is contained at the Russian Scientific Electron Library website:  http://elibrary.ru/item.asp?id=21092710

15.06.2015, 1564 просмотра.