Adaptation of CHO-K1 Cell Line to Suspension Culturing in Commercial Serum-Free Media

Автор: A.V. Petrov, N.V. Pigareva, B.V. Murashev, M.M. Karasev, and A.S. Simbirtsev

Страница: 50-58

Adaptation of CHO-K1 Cell Line to Suspension Culturing in Commercial Serum-Free Media

Biotekhnologiya, 2012, N 2, P. 50-58

UDC 57.086.83

Section:  “Biologicals Technology”

A.V. Petrov ^*,  N.V. Pigareva,  B.V. Murashev,  M.M. Karasev,  and  A.S. Simbirtsev

The State Research Institute for Highly Pure Biopreparations, Federal Medicobiological Agency of Russian Federation,  197110, St.-Petersburg Russia

e-mail:  avpetrov@hpb-spb.com

The current article describes the adaptation of CHO-K1 cells to the suspension growth in commercial serum-free media. The cells were successfully adapted in minibioreactors to four from ten tested media; however, the growth with doubling time less than 24 hours was only observed in two of them. The obtained suspension cells were shown to be transfectable in the serum-free medium.

Key words:  CHO-K1 cells,  serum-free medium,  suspension culturing.

The full English version of the article was published in “Biotechnology in Russia”, 2012, Issue 2, pp. 50-58 as A.V. Petrov, N.V. Pigareva, B.V. Murashev, M.M. Karasev, A.S. Simbirtsev “Adaptation of CHO-K1 Cell Line to Suspension Culturing in Commercial Serum-Free Media”.

It is contained at the Russian Scientific Electron Library website:  http://elibrary.ru/item.asp?id=21092709

15.06.2015, 3335 просмотров.