|
Russian Journal of Biotechnology
→
Articles archive
Archive 2012
→
N 2
→
Adaptation of CHO-K1 Cell Line to Suspension Culturing in Commercial Serum-Free Media
Adaptation of CHO-K1 Cell Line to Suspension Culturing in Commercial Serum-Free MediaАвтор: A.V. Petrov, N.V. Pigareva, B.V. Murashev, M.M. Karasev, and A.S. Simbirtsev Страница: 50-58
Adaptation of CHO-K1 Cell Line to Suspension Culturing in Commercial Serum-Free Media Biotekhnologiya, 2012, N 2, P. 50-58 UDC 57.086.83 Section: “Biologicals Technology”
A.V. Petrov *, N.V. Pigareva, B.V. Murashev, M.M. Karasev, and A.S. Simbirtsev The State Research Institute for Highly Pure Biopreparations, Federal Medicobiological Agency of Russian Federation, 197110, St.-Petersburg Russia e-mail: avpetrov@hpb-spb.com
The current article describes the adaptation of CHO-K1 cells to the suspension growth in commercial serum-free media. The cells were successfully adapted in minibioreactors to four from ten tested media; however, the growth with doubling time less than 24 hours was only observed in two of them. The obtained suspension cells were shown to be transfectable in the serum-free medium.
Key words: CHO-K1 cells, serum-free medium, suspension culturing.
The full English version of the article was published in “Biotechnology in Russia”, 2012, Issue 2, pp. 50-58 as A.V. Petrov, N.V. Pigareva, B.V. Murashev, M.M. Karasev, A.S. Simbirtsev “Adaptation of CHO-K1 Cell Line to Suspension Culturing in Commercial Serum-Free Media”. It is contained at the Russian Scientific Electron Library website: http://elibrary.ru/item.asp?id=21092709
15.06.2015, 3443 просмотра. |
ContactsPostal address: Tel: +7 (495) 315-08-01 Email: editor@genetika.ru |