Transgenic Tomato Plants as Producers of a Thaumatin II Supersweet Protein

Автор: A.P. Firsov, A.S. Pushin, I.V. Korneeva, and S.V. Dolgov

Страница: 43-49

Transgenic Tomato Plants as Producers of a Thaumatin II Supersweet Protein

Biotekhnologiya, 2012, N 2, P. 43-49

UDC 582.951.4:575.21:681.134.4:664

Section:  “Producers, Biology, Selection, and Gene Engineering”

A.P. Firsov ^{1, 2, *},  A.S. Pushin ^{1, 2, *},  I.V. Korneeva ²,  and  S.V. Dolgov ^{1, 2}

¹  The Shemyakin-and-Ovchinnikov Institute for Bioorganic Chemistry, Russ. Acad. Sci., Pushchino Branch,  142290, Pushchino, moskovskaya oblast Russia

²  The All-Russian Research Institute for Agricultural Biotechnology, Russ. Acad. Agric. Sci.,  127550, Moscow Russia

e-mail:  aspushin@rambler.ru,  aleksey_firsov@mail.ru

Tomato plants of Yalf cv. have been transformed with a gene for thaumatin II from Thaumatococcus daniellii Benth. The nucleotide sequence for the thaumatin II cDNA was cloned in a vector of pBI121 under the control of  CaMV 35S-promoter. The expression of the thaumatin II gene was detected in all studied transgenic lines. A quantitative estimation of the thaumatin II accumulation in fruits was performed by ELISA. The highest content of thaumatin in the transgenic tomato fruit (line 91) was 46,4±10,5 µg/mg of total soluble protein (4,6%). In the other studied lines, the thaumatin accumulation ranged from 17,6±6,1 to 41,3±12,3 µg/mg of total soluble protein (1,8%—4,1%). The fruits of transgenic plants had well-defined sweet taste with typical for thaumatin II long aftertaste. The tomato transgenic lines with the high expression levels can potentially be used as producers of thaumatin for food and pharmaceutical industries.

Key words:  sugar substitutes,  thaumatin,  tomato,  transgenic plants.

The full English version of the article was published in “Applied Biochemistry and Microbiology”, 2012, Vol. 48, Issue 9, pp. 746-751 as A. P. Firsov, A. S. Pushin, I. V. Korneeva, S. V. Dolgov “Transgenic tomato plants as supersweet protein thaumatin II producers”.

It is contained at the SpringerLink website:  http://link.springer.com/article/10.1134/S0003683812090025  and  at the Russian Scientific Electron Library website:  http://elibrary.ru/item.asp?id=20490423

DOI: 10.1134/S0003683812090025

15.06.2015, 1732 просмотра.