Putative GalP, XylE and FucP H+-symporters from Pantoea ananatis Are Capable of Glucose Transporting into Escherichia coli Cells

Автор: I.G. Andreeva, L.I. Golubeva, and J.I. Katashkina

Страница: 21-31

Putative GalP, XylE and FucP H⁺-symporters from Pantoea ananatis are capable of glucose transporting into Escherichia coli cells

Biotekhnologiya, 2012, N 2, P. 21-31

UDC 547.445.623:577.213.7

Section:  “Producers, Biology, Selection, and Gene Engineering”

I.G. Andreeva ^*,  L.I. Golubeva,  and  J.I. Katashkina

The Ajinomoto—Genetika Research Institute,  117545, Moscow Russia

e-mail:  irina_andreeva@agri.ru

The involvement of GalP low-affinity H⁺-symporters in the glucose uptake by Pantoea ananatis cells has been demonstrated. The putative galP, xylE and fucP genes from P. ananatis AJ13355, orthologs of the known E. coli genes for H⁺-symporters of D-galactose, D-xylose, and L-fucose, respectively, were cloned. It was confirmed that the constitutive expression of each of the cloned genes restored the deleted E. coli MG1655Δ(ptsHI-crr) strain growth on D-glucose. The constructed integrative cassettes providing the constitutive expression of the galP, xylE or fucP genes from P. ananatis could be exploited for optimization of glucose consumption in producing strains based on P. ananatis or E. coli.

Key words:  H⁺-symporters,  Pantoea ananatis,  PTS-alternative transporters,  soil carbohydrates.

The full English version of the article was published in “Applied Biochemistry and Microbiology”, 2013, Vol. 49, Issue 7, pp. 638-645 as I. G. Andreeva, L. I. Golubeva, J. I. Katashkina “Putative GalP, XylE, and FucP H⁺ symporters from Pantoea ananatis are capable of transporting glucose into Escherichia coli cells”.

It is contained at the SpringerLink website:  http://link.springer.com/article/10.1134/S0003683813070016  and  at the Russian Scientific Electron Library website:  http://elibrary.ru/item.asp?id=21883270

DOI: 10.1134/S0003683813070016

15.06.2015, 1613 просмотров.