Adaptation and Optimization of the Real-Time PCR Method for Microbiological Assay of Meat Stuffs

Автор: S.V. Kolotvina

Страница: 85-91

Adaptation and Optimization of the Real-Time PCR Method for Microbiological Assay of Meat Stuffs

Biotekhnologiya, 2012, N 3, P. 85-91

UDC 637.5.07

Section:  “Metrology, Standartization, and Control”

S.V. Kolotvina

The Moscow State University of Food Industry,  109316, Moscow Russia

e-mail:  kufiya@mail.ru

A possibility of using the methods of molecular genetics for estimation of sanitary-hygiene status of meat products have been investigated as a supplement to the traditional microbiological analysis. The optimum conditions for the functioning of test-systems for identification of starter cultures (Staphylococcus carnosus, Lactobacillus curvatus, Pediococcus pentosaceus) in fermented meat products by the real-time PCR were designed; the sensitivity of the method was 10³ CFU/ml. It was also established that the real-time PCR can be applied to the detection of Staphylococcus aureus, Escherichia coli, Proteus vulgaris, Salmonella typhimurium and Listeria monocytogenes bacteria species (all belonging to the sanitary-indicative microflora) in meat products. The specificity of the method was confirmed by the dependency of the PCR results on the added microorganism concentration.

Key words:  fermented meat products,  methods of molecular genetics,  primers,  probes,  real-time PCR,  sanitary-indicative microflora,  starter cultures.

The full English version of the article was published in “Biotechnology in Russia”, 2012, Issue 3, pp. 85-91 as S. V. Kolotvina “Adaptation and Optimization of the Real-Time PCR Method for Microbiological Assay of Meat Stuffs”.

It is contained at the Russian Scientific Electron Library website:  http://elibrary.ru/item.asp?id=21490728

25.05.2015, 1103 просмотра.