|
Russian Journal of Biotechnology
→
Articles archive
Archive 2012
→
N 3
→
Adaptation and Optimization of the Real-Time PCR Method for Microbiological Assay of Meat Stuffs
Adaptation and Optimization of the Real-Time PCR Method for Microbiological Assay of Meat StuffsАвтор: S.V. Kolotvina Страница: 85-91
Adaptation and Optimization of the Real-Time PCR Method for Microbiological Assay of Meat Stuffs Biotekhnologiya, 2012, N 3, P. 85-91 UDC 637.5.07 Section: “Metrology, Standartization, and Control”
S.V. Kolotvina The Moscow State University of Food Industry, 109316, Moscow Russia e-mail: kufiya@mail.ru
A possibility of using the methods of molecular genetics for estimation of sanitary-hygiene status of meat products have been investigated as a supplement to the traditional microbiological analysis. The optimum conditions for the functioning of test-systems for identification of starter cultures (Staphylococcus carnosus, Lactobacillus curvatus, Pediococcus pentosaceus) in fermented meat products by the real-time PCR were designed; the sensitivity of the method was 103 CFU/ml. It was also established that the real-time PCR can be applied to the detection of Staphylococcus aureus, Escherichia coli, Proteus vulgaris, Salmonella typhimurium and Listeria monocytogenes bacteria species (all belonging to the sanitary-indicative microflora) in meat products. The specificity of the method was confirmed by the dependency of the PCR results on the added microorganism concentration.
Key words: fermented meat products, methods of molecular genetics, primers, probes, real-time PCR, sanitary-indicative microflora, starter cultures.
The full English version of the article was published in “Biotechnology in Russia”, 2012, Issue 3, pp. 85-91 as S. V. Kolotvina “Adaptation and Optimization of the Real-Time PCR Method for Microbiological Assay of Meat Stuffs”. It is contained at the Russian Scientific Electron Library website: http://elibrary.ru/item.asp?id=21490728
25.05.2015, 1198 просмотров. |
ContactsPostal address: Tel: +7 (495) 315-08-01 Email: editor@genetika.ru |