Obtaining and Characteristics of a Recombinant Albumin-Binding Polypeptide from G Group Streptococcus for Diabetic Nephropathy Diagnostics

Автор: T.V. Gupalova, E.A. Bormotova, M.M. Gladilina, and A.A. Totolyan

Страница: 75-84

Obtaining and Characteristics of a Recombinant Albumin-Binding Polypeptide from G Group streptococcus for diabetic nephropathy Diagnostics

Biotekhnologiya, 2012, N 3, P. 75-84

UDC 577.112.824.825:577.2

Section:  “Metrology, Standartization, and Control”

T.V. Gupalova ^*,  E.A. Bormotova,  M.M. Gladilina,  and  A.A. Totolyan

The Research Institute for Experimental Medicine, Russ. Acad. Med. Sci., North-West Dept.,  197376, St.-Petersburg Russia

e-mail:  tvgupalova@rambler.ru

An A2 HSA-binding polypeptide from a DG13 strain of the group G streptococcus had been isolated from cow milk. It was shown, that the A2 polypeptide has higher HSA-binding activity relatively to the A1 polypeptide obtained from the G streptococcus of the human origin. An E. coli M 15 A2-producing strain allowing to obtain the A2 polypeptide with high yield and great HSA-binding activity was constructed. An essentially new method, REA (receptor-enzyme assay), for determining of microalbuminuria, a basic laboratory criterion of diabetic nephropathy pre-clinical diagnostics, was developed using the obtained A2 protein. This method is based on ELISA with the substitution of HSA-binding receptor protein for antibodies to HSA. We demonstrated that the design of a microarray for the assessment of microalbuminuria for screening and monitoring of diabetic nephropathy is also possible.

Key words:  diabetic nephropathy,  enzyme-linked immunosorbent assay (ELISA),  GA-module,  HSA-binding polypeptide,  microalbuminuria,  microarray,  recombinant protein.

25.05.2015, 1469 просмотров.