Design of a PCR-Test based on the gyrA Gene Sequence for Identification of Closely Relative Species of the Bacillus subtilis Group

Автор: L.N. Borshchevskaya, A.N. Kalinina, and S.P. Sineokii

Страница: 32-43

Design of a PCR-Test based on the gyrA Gene Sequence for Identification of Closely Relative Species of the Bacillus subtilis Group

Biotekhnologiya, 2012, N 3, P. 32-43

UDC 579.252.55.577.121.3

Section:  “Producers, Biology, Selection, and Gene Engineering”

L.N. Borshchevskaya ^*,  A.N. Kalinina,  and  S.P. Sineokii

The State Research Institute for Genetics and Selection of Industrial microorganisms (GosNIIgenetika),  117545, Moscow Russia

e-mail:  larbor3@rambler.ru

A method for the taxonomic identification of 7 close bacteria species of the Bacillus subtilis group (B. subtilis, B. amyloliquefaciens, B. licheniformis, B. vallismortis, B. atrophaeus, B. sonorensis, and B. mojavensis) using specific primers selected on the basis of the gyrA gene sequences has been developed. The effectiveness of the method for either pure cultures of type or collection and industrial bacterial strains of this group was demonstrated. The method seems to be applicable in principle to the detection of the B. subtilis group bacteria in mixed cultures.

Key words:  Bacillus subtilis,  gyrA gene,  identification,  species-specific primers.

The full English version of the article was published in “Applied Biochemistry and Microbiology”, 2013, Vol. 49, Issue 7, pp. 646-655 as L. N. Borshchevskaya, A. N. Kalinina, S. P. Sineokii “Design of a PCR test based on the gyrA gene sequence for the identification of closely related species of the Bacillus subtilis group”.

It is contained at the SpringerLink website:  http://link.springer.com/article/10.1134/S0003683813070028  and  at the Russian Scientific Electron Library website:  http://elibrary.ru/item.asp?id=21883259

DOI: 10.1134/S0003683813070028

25.05.2015, 1721 просмотр .