Assessment of Toxic Effect during Transient Expression of Genes for Proteases of Human Pathogens in HEK293 Line Cells

Автор: A.V. Shubin, N.A. Lunina, E.N. Shedova, M.P. Roshchina, I.V. Demidiuk, T.V. Vinogradova, E.P. Kopantsev, I.P. Chernov, and S.V. Kostrov

Страница: 82-88

Assessment of Toxic Effect during Transient Expression of Genes for Proteases of Human Pathogens in HEK293 Line Cells

Biotekhnologiya, 2012, N 4, P. 82-88

UDC 57.083.37

Section:  “Metrology, Standartization, and Control”

A.V. Shubin ¹,  N.A. Lunina ¹,  E.N. Shedova ¹,  M.P. Roshchina ¹,  I.V. Demidiuk ^{1, *},  T.V. Vinogradova ²,  E.P. Kopantsev ²,  I.P. Chernov ²,  and  S.V. Kostrov ¹

¹  The Institute for Molecular Genetics, Russ. Acad. Sci.,  123182, Moscow Russia

²  The Shemyakin-and-Ovchinnikov Institute for Bioorganic Chemistry, Russ. Acad. Sci.,  117997, Moscow Russia

e-mail:  duk@img.ras.ru

A method for the in vitro assessment of the toxic effect of some human diseases causes as a result of their genes expression in human cells has been suggested. The technique is based on the transient expression of the genes of interest in the HEK293 line cells. Using this approach, it was demonstrated for the first time that the expression of human hepatitis A virus 3C protease induces cell death. It was also shown that the expression of human poliovirus 2A protease results in cell death, whereas cathepsins B and D did not show any cytotoxic effect on the used culture.

Key words:  cell death,  cytotoxicity,  lipofection,  transient expression.

The full English version of the article was published in “Applied Biochemistry and Microbiology”, 2013, Vol. 49, Issue 9, pp. 750-755 as A. V. Shubin, N. A. Lunina, E. N. Shedova, M. P. Roshina, I. V. Demidyuk, T. V. Vinogradova, E. P. Kopantsev, I. P. Chernov, S. V. Kostrov “Evaluation of the toxic effects evoked by the transient expression of protease genes from human pathogens in HEK293 cells”.

It is contained at the SpringerLink website:  http://link.springer.com/article/10.1134/S0003683813090044  and  at the Russian Scientific Electron Library website:  http://elibrary.ru/item.asp?id=21888720

DOI: 10.1134/S0003683813090044

08.05.2015, 1362 просмотра.