Design of Polymeric Microspheres for Immunofluorescent Analysis

Автор: E.V. Volkova, I.A. Gritskova, S.A. Gusev, A.D. Lukashevich, A.A. Gusev, E.N. Levshenko, L.A. Zlydneva, and K.O. Sochilina

Страница: 74-81

Design of Polymeric Microspheres for Immunofluorescent Analysis

Biotekhnologiya, 2012, N 4, P. 74-81

UDC 541.64:543.426

Section:  “Metrology, Standartization, and Control”

E.V. Volkova ^{1, *},  I.A. Gritskova ¹,  S.A. Gusev ²,  A.D. Lukashevich ¹,  A.A. Gusev ²,  E.N. Levshenko ¹,  L.A. Zlydneva ¹,  and  K.O. Sochilina ³

¹  The Lomonosov Moscow State University for Fine Chemical Technologies,  119571, Moscow Russia

²  The Research Institute for Physicochemical Medicine,  119435, Moscow Russia

³  Synthesis of Polymeric Sorbents, Co.,  119361, Moscow Russia

e-mail:  voleko@gmail.com

Carboxylated polystyrene suspensions with narrow particle-size distribution have been synthesized by seed polymerization in a mixture containing organosilicone surfactants. The characteristics of the particles such as average particle diameter (5,8 μm), zeta-potential (–31,6 mV) and functional group quantity were determined. We have developed a technique that makes it possible to homogeneously stain the microspheres with a fluorescent dye across the whole body of their suspension and to reach various emission intensity. Mixtures of these microspheres might make these particles helpful for multiplex immunofluorescent bead-based analysis.

Key words:  immunofluorescent analysis,  narrow particle-size distribution,  organosilicone surfactant,  polystyrene microspheres,  seed polymerization.

The full English version of the article was published in “Biotechnology in Russia”, 2012, Issue 4, pp. 74-81 as E.V. Volkova, I.A. Gritskova, S.A. Gusev, A.D. Lukashevich, A.A. Gusev, E.N. Levchenko, L.A. Zlydneva, K.O. Sochilina “Design of Polymeric Microspheres for Immunofluorescent Analysis”.

It is contained at the Russian Scientific Electron Library website:  http://elibrary.ru/item.asp?id=21532468

08.05.2015, 1252 просмотра.