Immobilization of β-Fructofuranosidase on a Fibrous Carrier

Автор: O.L. Mescheriakova, O.Yu. Bozhko, G.P. Shuvaeva, and O.S. Korneeva

Страница: 58-62

Immobilization of β-fructofuranosidase on a fibrous Carrier

Biotekhnologiya, 2012, N 4, P. 58-62

UDC 577.152.321

Section:  “Biologicals Technology”

O.L. Mescheriakova ¹,  O.Yu. Bozhko ^{2, *},  G.P. Shuvaeva ²,  and  O.S. Korneeva ²

¹  The All-Russian Research Institute of feed industry,  394026, Voronezh Russia

²  The Voronezh State University of Engineering Technology,  394036, Voronezh Russia

e-mail:  olga_bojko2005@mail.ru,  gpshuv@mail.ru

An immobilized preparation of β-fructofuranosidase on a FIBAN A-6 fibrous ion-exchanger has been obtained. The optimum concentrations for the enzyme immobilization were selected. It was established that the immobilized preparation displays its maximum activity at pH 4,0 and 70°С. The β-fructofuranosidase retained the starting level of activity when stored for 6 months or used for 5 cycles.

Key words:  activity,  adsorption immobilization,  fibrous anion-exchanger,  β-fructofuranosidase.

The full English version of the article was published in “Biotechnology in Russia”, 2012, Issue 4, pp. 58-62 as O.L. Meshcheriakiva, O.Yu. Bozhko, G.P. Shuvaeva, O.S. Korneeva “Immobilization of β-fructofuranosidase on a fibrous Carrier”.

It is contained at the Russian Scientific Electron Library website:  http://elibrary.ru/item.asp?id=21532465

08.05.2015, 1495 просмотров.