Desalination of L-Valine Fermentation Broth by Electrodialysis through Ion-Exchange Membranes

Автор: А.Е. Aghajanyan, A.O. Tsaturyan, A.A. Vardanyan, and A.S. Saghiyan

Страница: 52-57

Desalination of L-Valine Fermentation Broth by Electrodialysis through Ion-Exchange Membranes

Biotekhnologiya, 2012, N 4, P. 52-57

UDC 577.112.382.6

Section:  “Biologicals Technology”

А.Е. Aghajanyan ^*,  A.O. Tsaturyan,  A.A. Vardanyan,  and  A.S. Saghiyan

The Research and Industrial Center Armbiotechnology, Natl. Acad. Sci. of Armenia,  0056, Yerevan Armenia

e-mail:  aghajanyanarmen@yahoo.com

A process of desalination by the electromembrane method of a permeate of L-valine culture liquid (CL) has been studied (the fermentation was carried out without the addition of chalk). The desalination was performed using a five-chamber electrodialyzer. Changes of dry matters (DM), pH, electroconductivity of the solution, as well as voltage in the circuit during the process of L-valine permeate desalination depending on time were determined at the ultimate current density. Kinetic migration curves for valine and concomitant amino acids through MK-40 cation-exchanging membrane during the desalination were plotted. The investigations showed that electrodialysis enabled to purify the L-valine permeate from mineral ions (the degree of desalination of 98,5%) and from basic and acidic amino acids with minimal losses of the target product (3,9%). The specific energy consumption for the transportation of ions through membranes during the  permeate desalination was 0,48 A/h/kg and the current efficiency was 85%. It was thus shown that a technologically accessible electromembrane method for desalination of culture liquids of amino acids and probably of other biologically active compounds can be developed.

Key words:  desalination,  electrodialysis,  permeate,  valine.

The full English version of the article was published in “Biotechnology in Russia”, 2012, Issue 4, pp. 52-57 as А.Е. Aghajanyan, A.O. Tsaturyan, A.A. Vardanyan, A.S. Saghiyan “Desalination of L-Valine Fermentation Broth by Electrodialysis through Ion-Exchange Membranes”.

It is contained at the Russian Scientific Electron Library website:  http://elibrary.ru/item.asp?id=21532464

08.05.2015, 1630 просмотров.