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Isolation of an A-1149 Antibiotic Complex from Producing Strain Culture Broth using Amberlite XAD-2 and Ultradispersed Carbon Sorbent (UDUS)
Isolation of an A-1149 Antibiotic Complex from Producing Strain Culture Broth using Amberlite XAD-2 and Ultradispersed Carbon Sorbent (UDUS)Автор: A.V. Timofeeva, O.A. Lapchinskaya, E.A. Stepashkina, E.G. Gladkikh, V.V. Pogozheva, L.A. Baratova, and G.S. Katrukha Страница: 45-51
Isolation of an A-1149 antibiotic complex from producing strain culture broth using Amberlite XAD-2 and ultradispersed carbon sorbent (UDUS) Biotekhnologiya, 2012, N 4, P. 45-51 UDC 577.181.3 Section: “Biologicals Technology”
A.V. Timofeeva 1, *, O.A. Lapchinskaya 2, E.A. Stepashkina 2, E.G. Gladkikh 2, V.V. Pogozheva 2, L.A. Baratova 1, and G.S. Katrukha 2 1 The Belozerskii Research Institute for Physicochemical Biology, Lomonosov Moscow State University, 119992, Moscow Russia 2 The Gause Institute of Novel Antibiotics, Russ. Acad. Med. Sci., 119021, Moscow Russia e-mail: timofeeva@belozersky.msu.ru
A novel UDUS and a known polysterene Amberlite XAD-2 adsorbents were applied for the first time to the extraction of an A-1149 antibiotic complex from the cultural broth of a Streptomyces sp. № 1149 producing strain. It was established by the method of thin-layer chromatography and following bioautography that unlike Amberlite, UDUS more selectively extracts the A-1149 antibiotic complex components with lipophylic characteristics and minimizes ninhydrin-positive admixtures in the preparation. This permits to essentially reduce the number of stages required for the isolation and purification of the novel antibiotic.
Key words: А-1149 antibiotic, Amberlite XAD-2, bioautography, culture liquid, native solution, Streptomyces sp. № 1149 producing strain, TLC, ultradispersed carbon sorbent (UDUS).
The full English version of the article was published in “Biotechnology in Russia”, 2012, Issue 4, pp. 45-51 as A.V. Timofeeva, O.A. Lapchinskaya, E.A. Stepashkina, E.G. Gladkikh, V.V. Pogozheva, L.A. Baratova, G.S. Katrukha “Isolation of an A-1149 antibiotic complex from producing strain culture broth using Amberlite XAD-2 and ultradispersed carbon sorbent (UDUS)”. It is contained at the Russian Scientific Electron Library website: http://elibrary.ru/item.asp?id=21532463
08.05.2015, 1985 просмотров. |
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