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Isolation and Properties of Cyclodextringlucanotransferase from Paenibacillus ehimensis VKM B-2680D (IB-739) Bacterial Strain
Isolation and Properties of Cyclodextringlucanotransferase from Paenibacillus ehimensis VKM B-2680D (IB-739) Bacterial StrainАвтор: P.Yu. Fedorova, E.A. Gilvanova, G.E. Aktuganov, and N.G. Usanov Страница: 31-38
Isolation and Properties of cyclodextringlucanotransferase from Paenibacillus ehimensis VKM B-2680D (IB-739) bacterial strain Biotekhnologiya, 2012, N 4, P. 31-38 UDC 577.152.2 Section: “Producers, Biology, Selection, and Gene Engineering”
P.Yu. Fedorova *, E.A. Gilvanova, G.E. Aktuganov, and N.G. Usanov The Institute for Biology, Ufa Research Center, Russ. Acad. Sci., 450054, Ufa Russia e-mail: millinariya@yandex.ru
A new extracellular cyclodextrin glucanotransferase (CGTase) ( EC 2.4.1.19) has been found out in a bacterial species of Paenibacillus ehimensis and characterized. The enzyme from the P. ehimensis VKM B-2680D (IB-739) isolate was partially purified from culture liquid by the adsorption on starch and column chromatography on DEAE-Sepharose. The CGTase was stable within the pH range 5,5—8,2 and temperature inferior to 60C. The enzyme showed the maximal cyclizing activity at 40—50°C and pH 5,5—6,5. According to the SDS-electrophoresis data, the enzyme has a molecular mass of ~ 75 kDa. The CGTase thermal stability could be slightly improved in the presence of
Key words: cyclodextringlucanotransferase, cyclodextrins, Paenibacillus ehimensis VKM B-2680D (IB-739).
The full English version of the article was published in “Biotechnology in Russia”, 2012, Issue 4, pp. 31-38 as P.Yu. Fedorova, E.A. Gil'vanova, G.E. Aktuganov, N.G. Usanov “Isolation and Properties of cyclodextrin Glucanotransferase from a Paenibacillus ehimensis VKM B-2680D (IB-739) bacteria strain”. It is contained at the Russian Scientific Electron Library website: http://elibrary.ru/item.asp?id=21532461
08.05.2015, 1829 просмотров. |
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