Isolation and Properties of Cyclodextringlucanotransferase from Paenibacillus ehimensis VKM B-2680D (IB-739) Bacterial Strain

Автор: P.Yu. Fedorova, E.A. Gilvanova, G.E. Aktuganov, and N.G. Usanov

Страница: 31-38

Isolation and Properties of cyclodextringlucanotransferase from Paenibacillus ehimensis VKM B-2680D (IB-739) bacterial strain

Biotekhnologiya, 2012, N 4, P. 31-38

UDC 577.152.2

Section:  “Producers, Biology, Selection, and Gene Engineering”

P.Yu. Fedorova ^*,  E.A. Gilvanova,  G.E. Aktuganov,  and  N.G. Usanov

The Institute for Biology, Ufa Research Center, Russ. Acad. Sci.,  450054, Ufa Russia

e-mail:  millinariya@yandex.ru

A new extracellular cyclodextrin glucanotransferase (CGTase) ( EC 2.4.1.19) has been found out in a bacterial species of Paenibacillus ehimensis and characterized. The enzyme from the P. ehimensis VKM B-2680D (IB-739) isolate was partially purified from culture liquid by the adsorption on starch and column chromatography on DEAE-Sepharose. The CGTase was stable within the pH range 5,5—8,2 and temperature inferior to 60C. The enzyme showed the maximal cyclizing activity at 40—50°C and pH 5,5—6,5. According to the SDS-electrophoresis data, the enzyme has a molecular mass of ~ 75 kDa. The CGTase thermal stability could be slightly improved in the presence of 10 мМ CaCl₂. The daily conversion of 5% potato starch to α-, β-, and γ-cyclodextrins under the optimum conditions was 40% with their mass ratio equal to 14,6:21,4:4,0, respectively.

Key words:  cyclodextringlucanotransferase,  cyclodextrins,  Paenibacillus ehimensis VKM B-2680D (IB-739).

The full English version of the article was published in “Biotechnology in Russia”, 2012, Issue 4, pp. 31-38 as P.Yu. Fedorova, E.A. Gil'vanova, G.E. Aktuganov, N.G. Usanov “Isolation and Properties of cyclodextrin Glucanotransferase from a Paenibacillus ehimensis VKM B-2680D (IB-739) bacteria strain”.

It is contained at the Russian Scientific Electron Library website:  http://elibrary.ru/item.asp?id=21532461

08.05.2015, 1800 просмотров.