A Novel Biocatalytic Process of N-Sub¬stituted Acrylamide Syhthesis

Автор: K.V. Lavrov, G.A. Larikova, and A.S. Yanenko

Страница: 26-30

A Novel Biocatalytic Process of N-Sub­stituted Acrylamide Syhthesis

Biotekhnologiya, 2012, N 4, P. 26-30

UDC 5.7.3.6

Section:  “Producers, Biology, Selection, and Gene Engineering”

K.V. Lavrov,  G.A. Larikova,  and  A.S. Yanenko ^*

The State Research Institute for Genetics and Selection of Industrial Microorganisms,  117545, Moscow Russia

e-mail:  yanenko@genetika.ru

An enzymatic synthesis of N-substituted acrylamides (N-isopropyl acrylamide and N,N-dimethylaminopropyl acrylamide) has been demonstrated for the first time. The Rhodococcus erythropolis 37 strain possessing an acylamidase activity was used as a source of enzyme, whereas water-dissolved acrylamide and isopropylamine/dimethylaminopropylamine served as substrates. The optimum conditions for the synthesis of acrylamide N-substitutes were determined using N-isopropyl acrylamide. The maximum yield of the product was obtained at pH 9,5—10,5, ratio of the substrates (acrylamide/isopropylamine) 1,3;1—2:1 and absolute value of substrate concentration of 8,0% (acrylamide) and 4,0% (isopropylamine). Up to 22 g/l of N-isopropyl acrylamide was synthesized under the above conditions.

Key words:  acrylic monomers,  acylamidase,  biocatalyst,  N-isopropyl acrylamide,  Rhodococcus erythropolis.

The full English version of the article was published in “Applied Biochemistry and Microbiology”, 2013, Vol. 49, Issue 8, pp. 702-705 as K. V. Lavrov, G. A. Larikova, A. S. Yanenko “Novel biocatalytic process of N -substituted acrylamide synthesis”.

It is contained at the SpringerLink website:  http://link.springer.com/article/10.1134/S0003683813080048  and  at the Russian Scientific Electron Library website:  http://elibrary.ru/item.asp?id=21889115

DOI: 10.1134/S0003683813080048

08.05.2015, 1749 просмотров.