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A Novel Biocatalytic Process of N-Sub¬stituted Acrylamide Syhthesis
A Novel Biocatalytic Process of N-Sub¬stituted Acrylamide SyhthesisАвтор: K.V. Lavrov, G.A. Larikova, and A.S. Yanenko Страница: 26-30
A Novel Biocatalytic Process of N-Substituted Acrylamide Syhthesis Biotekhnologiya, 2012, N 4, P. 26-30 UDC 5.7.3.6 Section: “Producers, Biology, Selection, and Gene Engineering”
K.V. Lavrov, G.A. Larikova, and A.S. Yanenko * The State Research Institute for Genetics and Selection of Industrial Microorganisms, 117545, Moscow Russia e-mail: yanenko@genetika.ru
An enzymatic synthesis of N-substituted acrylamides (N-isopropyl acrylamide and N,N-dimethylaminopropyl acrylamide) has been demonstrated for the first time. The Rhodococcus erythropolis 37 strain possessing an acylamidase activity was used as a source of enzyme, whereas water-dissolved acrylamide and isopropylamine/dimethylaminopropylamine served as substrates. The optimum conditions for the synthesis of acrylamide N-substitutes were determined using N-isopropyl acrylamide. The maximum yield of the product was obtained at pH 9,5—10,5, ratio of the substrates (acrylamide/isopropylamine) 1,3;1—2:1 and absolute value of substrate concentration of 8,0% (acrylamide) and 4,0% (isopropylamine). Up to 22 g/l of N-isopropyl acrylamide was synthesized under the above conditions.
Key words: acrylic monomers, acylamidase, biocatalyst, N-isopropyl acrylamide, Rhodococcus erythropolis.
The full English version of the article was published in “Applied Biochemistry and Microbiology”, 2013, Vol. 49, Issue 8, pp. 702-705 as K. V. Lavrov, G. A. Larikova, A. S. Yanenko “Novel biocatalytic process of N -substituted acrylamide synthesis”. It is contained at the SpringerLink website: http://link.springer.com/article/10.1134/S0003683813080048 and at the Russian Scientific Electron Library website: http://elibrary.ru/item.asp?id=21889115 DOI: 10.1134/S0003683813080048
08.05.2015, 1772 просмотра. |
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