Optimization of Fed-Batch Culturing of CHO Cells Expressing Recombinant Darbepoietin-alpha in Circuited Volume

Автор: A.A. Nurbakov, N.V. Lobanova, I.N. Savinova, R.R. Shukurov, N.V. Orlova, A.G. Mosina, L.V. Ermolina, E.N. Sautkina, R.A. Khamitov, and Yu.A. Seryogin

Страница: 55-65

Optimization of Fed-Batch Culturing of CHO Cells Expressing Recombinant Darbepoietin-alpha in Circuited Volume

Biotekhnologiya, 2012, N 5, P. 55-65

UDC 602.6:612

Section:  “Biologicals Technology”

A.A. Nurbakov ^{1, *},  N.V. Lobanova ¹,  I.N. Savinova ¹,  R.R. Shukurov ¹,  N.V. Orlova ¹,  A.G. Mosina ¹,  L.V. Ermolina ²,  E.N. Sautkina ²,  R.A. Khamitov ²,  and  Yu.A. Seryogin ¹

¹  Limited Liability Company “PHARMAPARK”,  117246, Moscow Russia

²  The State Research Institute for Genetics and Selection of Industrial Microorganisms,  117545, Moscow Russia

e-mail:  alfred.nurbakov@bioprocess.ru

The study of cell growth rate and productivity for recombinant darbepoietin-alpha-producing CHO-­based cell line in plates and shaker flasks with the addition of various feeds aimed at increasing in product yield has been performed. It was demonstrated that Efficient Feed B (Invitrogen) provides the maximum improvement of the process in terms of productivity. A possibility of scaling the cultivation process up to wave bioreactor with disposable bags was investigated; the product yield per one cultivation cycle was 65-fold increased as compared to the flask growing, the specific productivity of the cell culture remaining constant.

Key words:  bioreactor,  CHO cells,  darbepoietin-alpha,  fed-batch culturing,  feed,  optimization.

The full English version of the article was published in “Biotechnology in Russia”, 2012, Issue 5, pp. 55-65 as A.A. Nurbakov, N.V. Lobanova, I.N. Savinova, R.R. Shikurov, N.V. Orlova, A.G. Mosina, L.V. Ermolina, E.N. Sautkina, R.A. Khamitov, Yu.A. Seryogin “Optimization of Fed-Batch Culturing of CHO Cells Expressing Recombinant Darbepoietin-alpha in Circuited Volume”.

It is contained at the Russian Scientific Electron Library website:  http://elibrary.ru/item.asp?id=21532472

06.05.2015, 1910 просмотров.