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Optimization of Fed-Batch Culturing of CHO Cells Expressing Recombinant Darbepoietin-alpha in Circuited Volume
Optimization of Fed-Batch Culturing of CHO Cells Expressing Recombinant Darbepoietin-alpha in Circuited VolumeАвтор: A.A. Nurbakov, N.V. Lobanova, I.N. Savinova, R.R. Shukurov, N.V. Orlova, A.G. Mosina, L.V. Ermolina, E.N. Sautkina, R.A. Khamitov, and Yu.A. Seryogin Страница: 55-65
Optimization of Fed-Batch Culturing of CHO Cells Expressing Recombinant Darbepoietin-alpha in Circuited Volume Biotekhnologiya, 2012, N 5, P. 55-65 UDC 602.6:612 Section: “Biologicals Technology”
A.A. Nurbakov 1, *, N.V. Lobanova 1, I.N. Savinova 1, R.R. Shukurov 1, N.V. Orlova 1, A.G. Mosina 1, L.V. Ermolina 2, E.N. Sautkina 2, R.A. Khamitov 2, and Yu.A. Seryogin 1 1 Limited Liability Company “PHARMAPARK”, 117246, Moscow Russia 2 The State Research Institute for Genetics and Selection of Industrial Microorganisms, 117545, Moscow Russia e-mail: alfred.nurbakov@bioprocess.ru
The study of cell growth rate and productivity for recombinant darbepoietin-alpha-producing CHO-based cell line in plates and shaker flasks with the addition of various feeds aimed at increasing in product yield has been performed. It was demonstrated that Efficient Feed B (Invitrogen) provides the maximum improvement of the process in terms of productivity. A possibility of scaling the cultivation process up to wave bioreactor with disposable bags was investigated; the product yield per one cultivation cycle was 65-fold increased as compared to the flask growing, the specific productivity of the cell culture remaining constant.
Key words: bioreactor, CHO cells, darbepoietin-alpha, fed-batch culturing, feed, optimization.
The full English version of the article was published in “Biotechnology in Russia”, 2012, Issue 5, pp. 55-65 as A.A. Nurbakov, N.V. Lobanova, I.N. Savinova, R.R. Shikurov, N.V. Orlova, A.G. Mosina, L.V. Ermolina, E.N. Sautkina, R.A. Khamitov, Yu.A. Seryogin “Optimization of Fed-Batch Culturing of CHO Cells Expressing Recombinant Darbepoietin-alpha in Circuited Volume”. It is contained at the Russian Scientific Electron Library website: http://elibrary.ru/item.asp?id=21532472 06.05.2015, 1925 просмотров. |
ContactsPostal address: Tel: +7 (495) 315-08-01 Email: editor@genetika.ru |