Hydrolysis of Saccharomyces cerevisiae Cell Wall by Enzyme Complexes from Basidial Mushrooms

Автор: S.I. Ibragimova, O.V. Tikhonova, T.E. Tolstikhina, B.F. Vasilieva, A.P. Sinitsyn, N.V. Tsurikova, I.N. Zorov, and O.V. Efremenkova

Страница: 53-60

Hydrolysis of Saccharomyces cerevisiae Cell Wall by Enzyme Complexes from Basidial Mushrooms

Biotekhnologiya, 2012, N 6, P. 53-60

UDC 579.67

Section:  “Biologicals Technology”

S.I. Ibragimova ^{1, *},  O.V. Tikhonova ²,  T.E. Tolstikhina ²,  B.F. Vasilieva ²,  A.P. Sinitsyn ^{1, 3},  N.V. Tsurikova ⁴,  I.N. Zorov ^{3, *},  and  O.V.  Efremenkova ²

¹  The Bach Institute for Biochemistry, Russ. Acad. Sci,  119071, Moscow Russia

²  The Gause Research Institute for Novel Antibiotics, Russ. Acad. Med. Sci,,  119021, Moscow Russia

³  The Lomonosov Moscow State University, faculty of chemistry,  119991, Moscow Russia

⁴  The All-Russian Research Institute of Food Biotechnology, Russ. Acad. Agric. Sci,  111033, Moscow Russia

e-mail:  inzorov@mail.ru

It has been shown, that during submerged cultivation, edible basidiomycetes Pleurotus ostreatus (oyster mushroom) and Lentinus edodes (shiitake) form enzymes that hydrolyze cell wall of Saccharomyces cerevisiae (baker’s yeast). Using culture liquids of these basidial mushrooms, it was possible to release 84,3% and 86,9%, respectively, of protein from S. cerevisiae cells, the value being significantly higher than those obtained using the analyzed commercial enzyme preparations. The co-cultivation of one of two species of ascomycetes yeast (S. cerevisiae or Candida famata) with P. ostreatus showed that the latter completely inhibited the S. cerevisiae growth and, on the contrary, the growth of P. ostreatus was strongly inhibited during the co-cultivation with Candida famata indicating species-specific antagonistic relationships among the higher fungi. The hydrolytic activity in P. ostreatus significantly increased when the mushrooms were co-cultured with S. cerevisiae or vinasse containing dead yeast cells of this species was used as a nutrient medium.

Key words:  antagonistic relationships between higher mushrooms,  cell wall hydrolysis,  co-cultivation,  edible protein,  Pleurotus ostreatus and Lentinus edodes basidial mushrooms,  Saccharomyces cerevisiae and Candida famata ascomycetes yeast.

The full English version of the article was published in “Biotechnology in Russia”, 2012, Issue 6, pp. 53-60 as S.I. Ibragimova, O.V. Tikhonova, T.E. Tolstikhina, B.F. Vasileva, A.P. Sinitsyn, N.V. Tsurikova, I.N. Zorov, O.V. Efremenkova “Hydrolysis of Saccharomyces cerevisiae Cell Wall by Enzyme Complexes from Basidial Mushrooms”.

It is contained at the Russian Scientific Electron Library website:  http://elibrary.ru/item.asp?id=22959637

29.04.2015, 1583 просмотра.