A Method for Fluorescence Monitoring of Vital Human Skin Cell Models during Cultivation in Microbioreactor

Автор: E.V. Trushkin, I.A. Sergachov, N.P. Petrova, V.A. Petrov, A.Yu. Shkurnikov, U. Marx, N.G. Luzgina, and D.A. Sakharov

Страница: 91-96

A Method for Fluorescence Monitoring of Vital Human Skin Cell Models during Cultivation in Microbioreactor

Biotekhnologiya, 2013, N 1, P. 91-96

UDC 57.04, 57.041, 57.032

Section:  “Metrology, Standartization, and Control”

E.V. Trushkin ^{1, *},  I.A. Sergachov ¹,  N.P. Petrova ¹,  V.A. Petrov ²,  A.Yu. Shkurnikov,  U. Marx ³,  N.G. Luzgina ⁴,  and  D.A. Sakharov ¹

¹  The Research and Technical Center BioClinicum,  115088, Moscow Russia

²  The Research Center for General Pathology and Pathophysiology, Russ. Acad. Med. Sci.,  125315, Moscow Russia

³  Technische Universitt Berlin,  10623, Berlin Germany

⁴  The Scientific Center for Clinical and Experimental Medicine,  630117, Novosibirsk Russia

e-mail:  e.trushkin@bioclinicum.com

A noninvasive method using a fluorescent dye and an optical light guide system for the assessment of vital cell number in a human skin-modeling cell culture under the conditions of microbioreactor has been suggested. It was shown that the system is efficient in growing of various skin models (derma, epidermis or their mixed cultures). The used dye permitted to perform noninvasive measurements during two weeks. The data on the number of vital cells obtained by direct counting and the suggested method were similar within the error range.

Key words:  HaCaT,  laser spectrometry,  microbioreactor,  skin cell models.

The full English version of the article was published in “Biotechnology in Russia”, 2013, Issue 1, pp. 91-96 as E.V. Trushkin, I.A. Sergachov, N.P. Petrova, V.A. Petrov, A.Yu. Shkurnikov, U. Marx, N.G. Luzgina, and D.A. Sakharov “A Method for Fluorescence Monitoring of Vital Human Skin Cell Models during Cultivation in Microbioreactor”.

It is contained at the Russian Scientific Electron Library website:  http://elibrary.ru/item.asp?id=23682128

27.04.2015, 1687 просмотров.