Current Technologies for in vitro Testing of Medicines: Use of Microbioreactors

Автор: N.V. Senyavina, E.V. Trushkin, A.L. Rusanov, V.A. Petrov, A.Yu. Shkurnikov, U. Marx, and D.A. Sakharov

Страница: 51-58

Current Technologies for in vitro Testing of Medicines: Use of Microbioreactors

Biotekhnologiya, 2013, N 1, P. 51-58

UDC 57.087

Section:  “Biologicals Technology”

N.V. Senyavina ^{1, *},  E.V. Trushkin ¹,  A.L. Rusanov ¹,  V.A. Petrov ²,  A.Yu. Shkurnikov ¹,  U. Marx ³,  and  D.A. Sakharov ¹

¹  The Research-and-Technique Center BioClinicum,  115088, Moscow Russia

²  The Research Institute for General Pathology and Pathophysiology, Russ. Acad. Med. Sci.,  125315, Moscow Russia

³  Technische Universität Berlin,  10623, Berlin Germany

e-mail:  n.seniavina@bioclinicum.com

A role of microbioreactors in the optimization of the design of medicinal preparations has been reviewed. Microbioreactors are microfluidic systems containing modules for co-culturing of a few organospecific cell lines (OCCL) and capable of modeling the interrelations between OCCLs under the conditions of close to physiological liquid flow. The use of micribioreactors makes an alternative to the conventional methods for screening based on the application of cell cultures with following preclinical trials on animals. Cell culturing can provide information about therapeutical effects of a definite compound on target tissue rather than entire organism, whereas testing on animals cannot always be extrapolated onto human. Microbioreactors that model physiological interactions between organs permit to perform more effective screening of medicines and their combinations. When autological tissue is used, microbioreactors can be helpful for personalized medicine.

Key words:  drug discign,  microbioreactor,  microfluidics,  toxicity.

The full English version of the article was published in “Biotechnology in Russia”, 2013, Issue 1, pp. 51-58 as N.V. Senyavina, E.V. Trushkin, A.L. Rusanov, V.A. Petrov, A.Yu. Shkurnikov, U. Marx, and D.A. Sakharov “Current Technologies for in vitro Testing of Medicines: Use of Microbioreactors”.

It is contained at the Russian Scientific Electron Library website:  http://elibrary.ru/item.asp?id=21987105

27.04.2015, 1721 просмотр .