Optimization of Glycosylation Profile of Recombinant Interferon-Beta-1a Produced by СНО Cells in Wave-Induced Motion Bioreactor

Автор: N.V. Lobanova, A.A. Nurbakov, V.I. Aksenova, A.A. Chupyrkina, N.A. Shamonov, A.A. Klishin, L.V. Ermolina, E.N. Sautkina, R.A. Khamitov, and Yu.A. Seryogin

Страница: 55-66

Optimization of Glycosylation Profile of Recombinant Interferon-Beta-1a Produced by СНО Cells in Wave-Induced Motion Bioreactor

Biotekhnologiya, 2013, N 2, P. 55-66

UDC 602.6:612

Section:  “Biologicals Technology”

N.V. Lobanova ^{1, *},  A.A. Nurbakov ¹,  V.I. Aksenova ¹,  A.A. Chupyrkina ¹,  N.A. Shamonov ¹,  A.A. Klishin ¹,  L.V. Ermolina ²,  E.N. Sautkina ²,  R.A. Khamitov ²,  and  Yu.A. Seryogin ¹

¹  Limited Liability Company “Pharmapark”,  117246, Moscow Russia

²  The State Research Institute for Genetics and Selection of Industrial Microorganisms,  117545, Moscow Russia

e-mail:  natalya.lobanova@bioprocess.ru

Culturing conditions for a recombinant interferon-beta-1а producing cell line based on Chinese hamster ovary cells (CHO) and cumate expression system have been optimized. The addition of 10 mM galactose and 2 mM uridine to a media was shown to improve the glycosylation profile of the target protein. Scaling-up of the cultivation process to single-use wave-induced motion bioreactor allowed to maintain the cell growth rate, specific productivity and interferon-beta-1а glycosylation that are characteristic of the small-volume culturing.

Key words:  bioreactor,  CHO cells,  cultivation,  interferon-beta-1a,  glycosylation,  optimization.

The full English version of the article was published in “Biotechnology in Russia”, 2013, Issue 2, pp. 55-66 as N.V. Lobanova, A.A. Nurbakov, V.I. Aksenova, A.A. Chupyrkina, N.A. Shamonov, A.A. Klishin, L.V. Ermolina, E.N. Sautkina, R.A. Khamitov, and Yu.A. Seryogin “Optimization of Glycosylation Profile of Recombinant Interferon-Beta-1a Produced by СНО Cells in Wave-Induced Motion Bioreactor”.

It is contained at the Russian Scientific Electron Library website:  http://elibrary.ru/item.asp?id=21818759

10.04.2015, 1813 просмотров.