Design of stable cell line Producing recombinant darbepoetin alpha based on CHO cells

Автор: R.R. Shukurov, N.V. Lobanova, I.N. Savinova, I.G. Vorobyova, A.A. Nurbakov, L.V. Ermolina, N.V. Orlova, A.G. Mosina, L.P. Antonova, R.A. Khamitov, and Yu.A. Seryogin

Страница: 46-54

Design of stable cell line Producing recombinant darbepoetin alpha based on CHO cells

Biotekhnologiya, 2013, N 2, P. 46-54

UDC 573.6

Section:  “Producers, Biology, Selection, and Gene Engineering”

R.R. Shukurov ^{1, *},  N.V. Lobanova ¹,  I.N. Savinova ¹,  I.G. Vorobyova ¹,  A.A. Nurbakov ¹,  L.V. Ermolina ²,  N.V. Orlova ¹,  A.G. Mosina ¹,  L.P. Antonova ¹,  R.A. Khamitov ²,  and  Yu.A. Seryogin ¹

¹  The Limited Liability Company “PHARMAPARK”,  117246, Moscow Russia

²  The State Research Institute for Genetics and Selection of Industrial Microorganisms,  117545, Moscow Russia

e-mail:  anamezon@gmail.com

A stable cell line based on CHO cells that produces 100 mg per liter of culture medium of recombinant darbepoetin alpha with the target glycosylated isoforms effective yield of about 30% has been selected. The expression product of the cell line was characterized and compared to the originator (Aranesp, Amgen). It was shown that the obtained preparation contained all isoforms characteristic of the originator. The created cell line can be used for the development of industrial cultivation and purification scheme for the recombinant darbepoetin alpha production.

Key words:  cell line,  CHO cells,  darbepoetin,  erythropoetin,  expression,  transfection.

The full English version of the article was published in “Applied Biochemistry and Microbiology”, 2014, Vol. 50, Issue 9, pp. 812-818 as R. R. Shukurov, N. V. Lobanova, I. N. Savinova, I. G. Vorobyova, A. A. Nurbakov, L. V. Ermolina, N. V. Orlova, A. G. Mosina, L. P. Antonova, R. A. Khamitov, and Yu. A. Seryogin “Design of a stable cell line producing recombinant darbepoetin alpha based on CHO cells”.

It is contained at the SpringerLink website:  http://link.springer.com/article/10.1134/S0003683814090063

DOI: 10.1134/S0003683814090063

10.04.2015, 1809 просмотров.