The Multiplex Serodiagnosis of Infectious Diseases. Tools for Results Accounting

Автор: A.G. Poltavchenko, A.V. Уоrsh, S.A. Pyankov, A.M. Nikonov, G.N. Volkov, and N.A. Krivenchuk

Страница: 74-82

The Multiplex Serodiagnosis of Infectious Diseases. Tools for Results Accounting

Biotekhnologiya, 2013, N 4, P. 74-82

UDC 616.9:576.8.077

Section:  “Metrology, Standardization, and Control”

A.G. Poltavchenko ^{1, *},  A.V. Уоrsh ¹,  S.A. Pyankov ¹,  A.M. Nikonov ¹,  G.N. Volkov ¹,  and  N.A. Krivenchuk ²

¹  The State Scintific Center for Virology and Biothecnology Vector,  630559, Kol'tsovo, Novosibirskaya oblast Russia

²  ImDi, Ltd.,  630559, Kol'tsovo, Novosibirskaya oblast Russia

e-mail:  poltav@vector.nsc.ru,  apoltav@gmail.com

Data on a tool (computer) accounting of the results obtained using protein microarrays for the multiplex dot immunoassay of antibodies to antigens of rubella, measles and mumps in blood are represented. The results obtained by the above method and with the use of monospecific  ELISA kits were compared. It was shown, that the tool accounting raises the reliability and plausibility of the assessment of the results of dot immunoassay during examining of low-titer sera and protective levels of antibodies. The multiplex analysis is more rapid and economical in comparison with ELISA; it can be used under field conditions and in combination with tool accounting can serve as an effective method for the control of collective and individual immunity in order to correct the activity aimed at the protection of children from vaccine-controlled infections.

Key words:  measles virus,  multiplexed dot-immunoassay,  mumps virus,  rubella virus,  tool accounting of results,  vaccine-challenged immunity.

The full English version of the article was published in “Biotechnology in Russia”, 2013, Issue 4, pp. 74-82 as A.G. Poltavchenko, A.V. Уоrsh, S.A. Pyankov, A.M. Nikonov, G.N. Volkov, and N.A. Krivenchuk “The Multiplex Serodiagnosis of Infectious Diseases. Tools for Results Accounting”.

It is contained at the Russian Scientific Electron Library website:  http://elibrary.ru/item.asp?id=22952566

03.04.2015, 1710 просмотров.