Bacterial Degradation of a Mixture Obtained as a result of Chemical Modification of Polychlorinated Biphenyls by Polyethylene Glycols

Автор: D.O. Egorova, T.I. Gorbunova, M.G. Pervova, and V.A. Demakov

Страница: 56-64

Bacterial degradation of a mixture obtained as a result of chemical modification of polychlorinated biphenyls by polyethylene glycols

Biotekhnologiya, 2013, N 4, P. 56-64

UDC 579.222.2;579.87;579.66

Section:  “Ecology”

D.O. Egorova ^{1, *},  T.I. Gorbunova ²,  M.G. Pervova ²,  and  V.A. Demakov ¹

¹  The Institute for Ecology and Genetics of Microorganisms, Russ. Acad. Sci., Ural Branch,  614081, Perm' Russia

²  Postovskii Institute for Organic Synthesis, Russ. Acad. Sci., Ural Branch,  620990, Ekaterinburg Russia

e-mail:  daryao@rambler.ru

Polychlorinated biphenyls (PCBs) of Sovol tradename, toxic industrial wastes, have been subjected to chemical treatment by polyethylene glycols (PEGs) and potassium hydroxide. As a result of the interaction of Sovol with various molecular mass PEGs (MM_PEG-4 ~ 200, MM_PEG-22 ~ 1000),  water-soluble mixtures of C1 and C2 containing mono(polyethylene glycol)oxy-derivatives (PCB-PEG-4 and PCB-PEG-22) polychlorobiphenylols and unreacted PCB congeners (PCB 44, PCB 47, PCB 49, PCB 52, PCB 66) were obtained. It was shown for the first time that the C1 and C2 mixtures are susceptible to bacterial degradation without their fractionation. According to the gas-liquid chromatography with flame-ionization and mass-spectrometric detection, a Rhodococcus wratislaviensis KT112-7 strain degraded all the chemical compounds occurring in the mixtures. It was found out from a 5-day experiment that the KT112-7 strain decomposes the mono(polyethylene glycol)oxy-derivatives completely (by 100%) and polychlorobiphenylols and PCB congeners by 90—95% in the C1 and C2 mixtures. the culture medium failed to contain the transformation products, whereas free chlorine ions were accumulated (72—94% of the maximum possible amount). Thus, the use of the chemical modification and consecutive bacterial degradation provided an effective destruction of technical PCB mixtures with a great content of high-chlorinated congeners.

Key words:  biological degradation,  chemical treatment,  congeners,  polychlorinated biphenyls,  polyethylene glycols,  Rhodococcus,  utilization.

The full English version of the article was published in “Applied Biochemistry and Microbiology”, 2014, Vol.50, Issue 7, pp. 722-729 as D. O. Egorova, T. I. Gorbunova, M. G. Pervova, and V. A. Demakov “Bacterial degradation of a mixture obtained through the chemical modification of polychlorinated biphenyls by polyethylene glycols”.

It is contained at the SpringerLink website:  http://link.springer.com/article/10.1134/S0003683814070023

DOI: 10.1134/S0003683814070023

03.04.2015, 1714 просмотров.