Effect of Various Additional Components on the Human Recombinant Erythropoietin Production and Sialylation Profile in CHO-Epo-2 Industrial Cell Line

Автор: N.V. Orlova, L.P. Antonova, N.V. Lobanova, A.G. Mosina, and L.V. Ermolina

Страница: 48-55

Effect of Various additional components on the human recombinant erythropoietin production and sialylation profile in CHO-Epo-2 Industrial cell line

Biotekhnologiya, 2013, N 4, P. 48-55

UDC 604.4:602.4

Section:  “Biologicals Technology”

N.V. Orlova ¹,  L.P. Antonova ²,  N.V. Lobanova ²,  A.G. Mosina ²,  and  L.V. Ermolina ^{1, *}

¹  The State Research Institute for Genetics and Selection of Industrial Microorganisms,  117545, Moscow Russia

²  Limited Liability Company “Pharmapark”,  117246, Moscow Russia

e-mail:  ermolina.lv@mail.ru

In this work, we have screened factors potentially affecting the level of productivity and sialylation profile of EPO in СHO-Epo-2 industrial line (amino acids, vitamins, saccharides, and others). An impulse application of sodium butyrate, an inhibitor of histone deacetylase, was shown to induce the largest increase (more than by 1,5 times) in the target protein content with retaining of the optimal isoform sialylation profile in culture liquid.

Key words:  erythropoietin,  productivity,  roller cultivation,  sialylation profile,  sodium butyrate.

The full English version of the article was published in “Biotechnology in Russia”, 2013, Issue 4, pp. 48-55 as N.V. Orlova, L.P. Antonova, N.V. Lobanova, A.G. Mosina, and L.V. Ermolina “Effect of Various additional components on the human recombinant erythropoietin production and sialylation profile in CHO-Epo-2 Industrial cell line”.

It is contained at the Russian Scientific Electron Library website:  http://elibrary.ru/item.asp?id=22952565

02.04.2015, 1635 просмотров.