Expression of Xyloglucanase sp-Xeg Gene from Penicillium canescens Accelerates Growth and Rooting in Transgenic Aspen Plants

Автор: E.O. Vidyagina, Yu.A. Kovalitskaya, D.S. Loginov, O.V. Koroleva, and K.A. Shestibratov

Страница: 39-47

Expression of xyloglucanase sp-Xeg Gene from Penicillium canescens Accelerates Growth and Rooting in Transgenic Aspen Plants

Biotekhnologiya, 2013, N 4, P. 39-47

UDC 630;630*165.314,5;631.526.321

Section:  “Producers, Biology, Selection, and Gene Engineering”

E.O. Vidyagina ^{1, 3, *},  Yu.A. Kovalitskaya ^{1, 3},  D.S. Loginov ²,  O.V. Koroleva ²,  and  K.A. Shestibratov ^{1, 3, *}

¹  The Shemyakin-and-Ovchinnikov Institute of Bioorganic Chemistry, russ. Acad. Sci., Pushchino Branch,  142290, Pushchino, Moskovskaya oblast Russia

²  The Bach Institute of Biochemistry, russ. Acad. Sci.,  119071, Moscow Russia

³  The Pushchino State Institute of Natural Science,  142290, Pushchino, Moskovskaya oblast Russia

e-mail:  vidjagina@mail.ru,  schestibratov@fibkh.serpukhov.su

The analysis of the properties of transgenic clones of aspen (Populus tremula) containing an sp-Xeg recombinant gene for xyloglucanase of Penicillium canescens has shown complex modifications in the wood composition and phenotype of the plants. Biometric analysis revealed an increase in the height of the transgenic plants as compared to control: the enhancement of the shoot size by 24,8%, 25% and 26% was observed for the PtXIVXeg1a, PtXVXeg1a, and PtXVXeg1b lines, respectively. The number of internodes was also enhanced in some transgenic clones. For the first time, modifications in plant rhizogenesis due to the occurrence of the recombinant gene of xyloglucanase were shown. In 13 of 25 lines, the efficiency of rooting in vitro exceeded the control value. The maximum rooting efficiency was observed in the PtXVXeg1a line (by 3,2 times higher than in control). The reliable increase in the mass of the root system in greenhouse for 8 of  25 clones was by 20% to 52% higher than the control value. All the lines were shown to contain less pentosans in wood. The data on the activity of xyloglucanase and pentosan content correlated in general with the phenotypic modifications.

Key words:  activity of xyloglucanase,  efficiency of rooting,  pentosans,  Populus tremula.

The full English version of the article was published in “Applied Biochemistry and Microbiology”, 2014, Vol. 50, Issue 7, pp. 701-708 as E. O. Vidyagina, Yu. A. Kovalitskaya, D. S. Loginov, O. V. Koroleva, and K. A. Shestibratov “Expression of xyloglucanase sp-Xeg gene from Penicillium canescens accelerates growth and rooting in transgenic aspen plants”.

It is contained at the SpringerLink website:  http://link.springer.com/article/10.1134/S0003683814070060

DOI: 10.1134/S0003683814070060

02.04.2015, 1763 просмотра.