Study on Immune Response to Mice Immunization by Recombinant Plasmids Incorporated in Biodegradable Microcapsules

Автор: V.I. Balysheva, S.Yu. Belov, N.N. Vlasova, O.V. Kapustina, O.E. Selina, I.R. Imatdinov, and E.A. Markvicheva

Страница: 71-77

Study on Immune Response to Mice Immunization by Recombinant Plasmids Incorporated in Biodegradable Microcapsules

Russian Journal of Biotechnology, 2013, N 5, P. 71-77

UDC 619:616.98:578.832.1:577.2

Section:  “Utilization of Biopreparations”

V.I. Balysheva ^{1, *},  S.Yu. Belov ¹,  N.N. Vlasova ^{1, *},  O.V. Kapustina ¹,  O.E. Selina ²,  I.R. Imatdinov ¹,  and  E.A. Markvicheva ²

¹  The All-Russian Research Institute for Veterinary Virology and Microbiology, Russ. Acad. Agric. Sci,  601120, Pokrov, Petushinskii region, Vladimirskaya oblast Russia

²  The Shemyakin-and-Ovchinnikov Institute for Bioorganic Chemistry, Russ. Acad. Sci.,  Moscow Russia

e-mail:  balyvi@rambler.ru,  vlanany@yandex.ru

Polyelectrolyte microcapsules of various compositions (chitosan/k-carrageenan, DEAE-dextran/κ-carrageenan and alginate/poly-L-lysine) containing four encapsulated in them plasmids have been prepared. pInfHA and pInfNP micro-encapsulated plasmids containing various genome fragments of the H5 and H7 influenza viruses, respectively, induced a partial resistance (55—60%) to the infection with virulent strains of the appropriate influenza viruses after mice immunization. The level of virus-­specific antibody response to the introduction of the same amount of micro-encapsulated pInfHA and pInfNP plasmids was dependent on the composition of microcapsules. The inoculation to mice of immobilized microspheres with the encapsulated in them pTZ57R/Gc and pTZ57R/Gn plasmids containing full-length insertions of genes encoding the Gn and Gc glycoproteins of Rift Valley fever virus provided a twofold increased titer of virus-specific antibodies as compared to unencapsulated plasmids.

Key words:  antibodies,  influenza virus,  microencapsulation,  recombinant plasmids,  Rift Valley fever virus.

01.04.2015, 1612 просмотров.