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Study on Physicochemical Characteristics of Biocatalysts with Thermostable Lipase Activity and Final Products of Triglyceride Ester Interchange
Study on Physicochemical Characteristics of Biocatalysts with Thermostable Lipase Activity and Final Products of Triglyceride Ester InterchangeАвтор: G.A. Kovalenko, L.V. Perminova, A.B. Beklemishev, and V.I. Tkachenko Страница: 35-50
Study on Physicochemical Characteristics of Biocatalysts with Thermostable Lipase Activity and Final Products of Triglyceride Ester Interchange Russian Journal of Biotechnology, 2013, N 6, P. 35-50 UDC 577.154.2 + 542.952 + 579.22 Section: “Biologicals Technology”
G.A. Kovalenko 1, 2, *, L.V. Perminova 1, A.B. Beklemishev 3, and V.I. Tkachenko 3 1 The Boreskov Institute for Catalysis, Russ. Acad. Sci., Siberian Branch, 630090, Novosibirsk Russia 2 The Novosibirsk State University, 630090, Novosibirsk Russia 3 The Research Institute for Biochemistry, Russ. Acad. Med. Sci., Siberian Branch, 630117, Novosibirsk Russia е-mail: galina@catalysis.ru
Physicochemical properties of multicomponent biocatalysts for triglyceride ester interchange prepared by the inclusion in xerogel of silicon dioxide of partly or completely destructed cells of a recombinant rE.coli/lip strain that produces a thermostable lipase from Thermomyces lanuginosus have been studied. A function and optimum contents of biocatalyst-introduced components (whole cells or cell lysates, water, water-retaining agents and nanostructured carbon forms (carbon nanotubes, nanospheres)) were investigated. The optimum composition of the biocatalysts prepared on the basis of rE.coli/lip cell lysates with enzymatic activity in water-free media was selected. When butter-fat mixtures are subjected to ester interchange in a flow-type packed-bed reactor, the time of half-inactivation of the prepared biocatalysts was ~70 h at 70—75°C.
Key words: cell lysates, ester interchange of triglycerides, inclusion in silicon dioxide xerogel, recombinant thermostable lipase, whole-cell biocatalysts.
31.03.2015, 1568 просмотров. |
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