Study on Physicochemical Characteristics of Biocatalysts with Thermostable Lipase Activity and Final Products of Triglyceride Ester Interchange

Автор: G.A. Kovalenko, L.V. Perminova, A.B. Beklemishev, and V.I. Tkachenko

Страница: 35-50

Study on Physicochemical Characteristics of Biocatalysts with Thermostable Lipase Activity and Final Products of Triglyceride Ester Interchange

Russian Journal of Biotechnology, 2013, N 6, P. 35-50

UDC 577.154.2 + 542.952 + 579.22

Section:  “Biologicals Technology”

G.A. Kovalenko ^{1, 2, *},  L.V. Perminova ¹,  A.B. Beklemishev ³,  and  V.I. Tkachenko ³

¹  The Boreskov Institute for Catalysis, Russ. Acad. Sci., Siberian Branch,  630090, Novosibirsk Russia

²  The Novosibirsk State University,  630090, Novosibirsk Russia

³  The Research Institute for Biochemistry, Russ. Acad. Med. Sci., Siberian Branch,  630117, Novosibirsk Russia

е-mail:  galina@catalysis.ru

Physicochemical properties of multicomponent biocatalysts for triglyceride ester interchange prepared by the inclusion in xerogel of silicon dioxide of partly or completely destructed cells of a recombinant rE.coli/lip strain that produces a thermostable lipase from Thermomyces lanuginosus have been studied. A function and optimum contents of biocatalyst-introduced components (whole cells or cell lysates, water, water-retaining agents and nanostructured carbon forms (carbon nanotubes, nanospheres)) were investigated. The optimum composition of the biocatalysts prepared on the basis of rE.coli/lip cell lysates with enzymatic activity in water-free media was selected. When butter-fat mixtures are subjected to ester interchange in a flow-type packed-bed reactor, the time of half-inactivation of the prepared biocatalysts was ~70 h at 70—75°C.

Key words:  cell lysates,  ester interchange of triglycerides,  inclusion in silicon dioxide xerogel,  recombinant thermostable lipase,  whole-cell biocatalysts.

31.03.2015, 1554 просмотра.