A Novel Saccharomyces cerevisiae-G Strain Possessing Inulinase Activity and Good Baking Characteristics

Автор: G.G. Sokolenko, and N.A. Karpechenko

Страница: 18-22

A Novel Saccharomyces cerevisiae-G Strain Possessing Inulinase Activity and Good Baking Characteristics

Russian Journal of Biotechnology, 2013, N 6, P. 18-22

UDC 60: 577.124

Section:  “Producers, Biology, Selection, and Gene Engineering”

G.G. Sokolenko ^{1, *},  and  N.A. Karpechenko ²

¹  The Peter I Voronezh State Agrarian University,  394087, Voronezh Russia

²  The Voronezh State University,  394006, Voronezh Russia

A new Saccharomyces cerevisiae-G strain has been shown to have an inulinase activity, synthesizing both intra- and extracellular inulinases with a significant prevalence of the first. The strain also demonstrates high baking properties (lifting force 38—40 min; maltase activity of 32 min; high osmotic resistance, biomass productivity, and others). Using RT-PCR, it was confirmed that the inulinase gene expression occurs in the strain and depends on used carbon source.

Key words:  expression,  inulinase,  PCR,  primers,  regulation,  Saccharomyces cerevisiae,  yeast.

31.03.2015, 1631 просмотр .