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A Novel Saccharomyces cerevisiae-G Strain Possessing Inulinase Activity and Good Baking Characteristics
A Novel Saccharomyces cerevisiae-G Strain Possessing Inulinase Activity and Good Baking CharacteristicsАвтор: G.G. Sokolenko, and N.A. Karpechenko Страница: 18-22
A Novel Saccharomyces cerevisiae-G Strain Possessing Inulinase Activity and Good Baking Characteristics Russian Journal of Biotechnology, 2013, N 6, P. 18-22 UDC 60: 577.124 Section: “Producers, Biology, Selection, and Gene Engineering”
G.G. Sokolenko 1, *, and N.A. Karpechenko 2 1 The Peter I Voronezh State Agrarian University, 394087, Voronezh Russia 2 The Voronezh State University, 394006, Voronezh Russia
A new Saccharomyces cerevisiae-G strain has been shown to have an inulinase activity, synthesizing both intra- and extracellular inulinases with a significant prevalence of the first. The strain also demonstrates high baking properties (lifting force 38—40 min; maltase activity of 32 min; high osmotic resistance, biomass productivity, and others). Using RT-PCR, it was confirmed that the inulinase gene expression occurs in the strain and depends on used carbon source.
Key words: expression, inulinase, PCR, primers, regulation, Saccharomyces cerevisiae, yeast.
31.03.2015, 1643 просмотра. |
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