Growth Characteristics and Darbepoietin Production by CHO Cell lines in Chemically Defined Serum and Prolein-free Hybris-1-CHO Medium

Автор: V.Yu. Tabakov, Yu.A. Seryogin, I.V. Chestkov, I.N. Savinova, A.A. Klishin, Yu.V. Shchepkina, and V.V. Chestkov

Страница: 78-82

Growth Characteristics and Darbepoietin Production by CHO Cell lines in Chemically Defined Serum and Prolein-free Hybris-1-CHO Medium

Biotekhnologiya, 2015, N 5, P. 78-82

UDC 57.085.23

Section:  “Biologicals Technology”

V.Yu. Tabakov ^{1, 2, *},  Yu.A. Seryogin ³,  I.V. Chestkov ²,  I.N. Savinova ³,  A.A. Klishin ³,  Yu.V. Shchepkina ^{1, 2},  and  V.V. Chestkov ^{1, 2}

¹  The Research Centre for Medical Genetics (RCMG),  Moscow, 115478 Russia

²  PanEco, Ltd., Research&Production Biomedical Company,  Moscow, 115522 Russia

³  The Limited Liability Company Pharmapark,  Moscow, 117246 Russia

e-mail:  tabakov@paneco-ltd.ru

A chemically defined medium Hybris-1-CHO designed for suspension culturing and recombinant protein production by CHO cell lines has been characterized. The two-stage character of cell growth and basic biochemical parameters was demonstrated in both batch and fed-batch experiments with the CHO-S model cell line. The data on the culture growth rate, its morphology, concentrations of key metabolites, and also culturing characteristics of the medium and darbepoetin productivity of the cultures on Hybris-1-CHO in comparison with a few commercial media permit to regard the designed medium as applicable to maintaining of protein biotechnological production.

Key words:  CHO-S;  darbepoetin;  recombinant protein;  serum-free medium;  suspension culturing.

20.05.2016, 1358 просмотров.