Construction of Transgenic Rape (Brassica napus L.) Overexpressing ARGOS-LIKE Gene from Arabidopsis thaliana using the Floral Dip Method

Автор: E.V. Mikhaylova, B.R. Kuluev

Страница: 49-58

Construction of Transgenic Rape (Brassica napus L.) Overexpressing ARGOS-LIKE Gene from Arabidopsis thaliana using the Floral Dip Method

Biotekhnologiya, 2015, N 5, P. 49-58

UDC 581.1:577.214.625:578.853

Section:  “Producers, Biology, Selection, and Gene Engineering”

E.V. Mikhaylova 1^{, *},  B.R. Kuluev ^{1, 2}

¹  The Bashkir State University,  Ufa, 450076 Russia

²  The Institute of Biochemistry and Genetics, Ufa Sci. Center of RAS,  Ufa, 450054 Russia

e-mail:  mikhele@list.ru

In this paper, we present transgenic rape plants overexpressing the ARGOS-LIKE gene from Arabidopsis thaliana that have been obtained by the floral dip method. The transformation success rate for rape cultivars of Hanna and Ratnik averaged at 10%. In comparison with control wild-type plants, transgenic plants showed the greater organ size: 27% in the stem length, 58% in the leaf length, 40% in the leaf width, 22% in corolla diameter, 23% in seed volume and 29% in seed weight. The lower epidermis cell area in transgenic plants increased on average by 18%. It was shown that the ARGOS-LIKE gene from A. thaliana along with the promoter of dahlia mosaic virus can be used for the enlargement of organ size in rape.

Key words:  ARGOS-LIKE gene,  Brassica napus L.,  dahlia mosaic virus promoter,  floral dip,  rape,  transgenic plants.

20.05.2016, 1416 просмотров.