A Role of Proteins of the Outer Membrane of Shewanella oneidensis MR-1 Bacteria in Formation and Stabilization of Silver Sulfide Nanoparticles

Автор: T.A. Voeikova, A.S. Shebanova, Yu.D. Ivanov, A.L. Kaysheva, L.M. Novikova, O.A. Zhuravliova, V.V. Shumyantseva, K.V. Shaitan, M.P. Kirpichnikov, and V.G. Debabov

Страница: 41-48

A Role of Proteins of the Outer Membrane of Shewanella oneidensis MR-1 Bacteria in Formation and Stabilization of Silver Sulfide Nanoparticles

Biotekhnologiya, 2015, N 5, P. 41-48

UDC 53.086

Section:  “Producers, Biology, Selection, and Gene Engineering”

T.A. Voeikova ^{1, *},  A.S. Shebanova ²,  Yu.D. Ivanov ³,  A.L. Kaysheva ³,  L.M. Novikova ¹,  O.A. Zhuravliova ^{1, 5},  V.V. Shumyantseva ³,  K.V. Shaitan ^{2, 4},  M.P. Kirpichnikov ²,  and  V.G. Debabov ¹

¹  The State Research Institute for Genetics and Selection of Industrial Microorganisms,  Moscow, 117545 Russia

²  The Lomonosov Moscow State University,  Moscow, 119991 Russia

³  The Orekhovich Institute for Biomedical Chemistry, Russ. Acad. Med. Sci.,  Moscow, 112343 Russia

⁴  The Semenov Institute of Chemical Physics, Russian Academy of Sciences,  Moscow, 119991 Russia

⁵  The Research and Education Center Nanotechnologies, People’s Friendship University,  Moscow, 117198 Russia

e-mail:  voeikova.tatyana@yandex.ru

Preparations of Ag₂S nanoparticles obtained in equimolar AgNO₃ and Na₂S₂O₃ aqueous solutions in the presence of Shewanella oneidensis MR-1 bacteria cells have been shown to be stable in aqueous solutions. Proteins absorbed on the surface of these nanoparticles were identified for the first time by the MALDI TOF/TOF method. Among them, multi-heme cytochromes MtrC and OmcA, as well as a MtrB membrane protein porin that forms a complex with these cytochromes on the outer cell membrane were detected. It was shown that in the absence of the cells, an insoluble precipitate consisting of agglomerated Ag₂S nanoparticles with broad size distribution was formed. The role of the identified proteins in the mechanism of the formation and stabilization of the Ag₂S nanoparticles in this system is discussed.

Key words:  biosynthesis,  membrane proteins,  nanoparticles,  Shewanella oneidensis MR-1.

20.05.2016, 1291 просмотр .