Russian Journal of Biotechnology Articles archive Archive 2015 N 5 A Role of Proteins of the Outer Membrane of Shewanella oneidensis MR-1 Bacteria in Formation and Stabilization of Silver Sulfide Nanoparticles

A Role of Proteins of the Outer Membrane of Shewanella oneidensis MR-1 Bacteria in Formation and Stabilization of Silver Sulfide Nanoparticles

Автор: T.A. Voeikova, A.S. Shebanova, Yu.D. Ivanov, A.L. Kaysheva, L.M. Novikova, O.A. Zhuravliova, V.V. Shumyantseva, K.V. Shaitan, M.P. Kirpichnikov, and V.G. Debabov

Страница: 41-48

 

A Role of Proteins of the Outer Membrane of Shewanella oneidensis MR-1 Bacteria in Formation and Stabilization of Silver Sulfide Nanoparticles

Biotekhnologiya, 2015, N 5, P. 41-48

UDC 53.086

Section:  “Producers, Biology, Selection, and Gene Engineering”

 

T.A. Voeikova 1, *,  A.S. Shebanova 2,  Yu.D. Ivanov 3,  A.L. Kaysheva 3,  L.M. Novikova 1,  O.A. Zhuravliova 1, 5,  V.V. Shumyantseva 3,  K.V. Shaitan 2, 4,  M.P. Kirpichnikov 2,  and  V.G. Debabov 1

1  The State Research Institute for Genetics and Selection of Industrial Microorganisms,  Moscow, 117545 Russia

2  The Lomonosov Moscow State University,  Moscow, 119991 Russia

3  The Orekhovich Institute for Biomedical Chemistry, Russ. Acad. Med. Sci.,  Moscow, 112343 Russia

4  The Semenov Institute of Chemical Physics, Russian Academy of Sciences,  Moscow, 119991 Russia

5  The Research and Education Center Nanotechnologies, People’s Friendship University,  Moscow, 117198 Russia

e-mail:  voeikova.tatyana@yandex.ru

 

Preparations of Ag2S nanoparticles obtained in equimolar AgNO3 and Na2S2O3 aqueous solutions in the presence of Shewanella oneidensis MR-1 bacteria cells have been shown to be stable in aqueous solutions. Proteins absorbed on the surface of these nanoparticles were identified for the first time by the MALDI TOF/TOF method. Among them, multi-heme cytochromes MtrC and OmcA, as well as a MtrB membrane protein porin that forms a complex with these cytochromes on the outer cell membrane were detected. It was shown that in the absence of the cells, an insoluble precipitate consisting of agglomerated Ag2S nanoparticles with broad size distribution was formed. The role of the identified proteins in the mechanism of the formation and stabilization of the Ag2S nanoparticles in this system is discussed.

 

Key words:  biosynthesis,  membrane proteins,  nanoparticles,  Shewanella oneidensis MR-1.

 

20.05.2016, 1334 просмотра.

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