Isolation and Purification of Bioinhibitory Product from Culture Liquid of Lacticacid Bacteria Lactobacillus acidophilus 1991 VKPM 6257 and Lactobacillus rhamnosus INMIA 9614

Автор: A.E. Aghajanyan, F.N. Tkhruni, G.Zh. Hovhannisyan, K.I. Yeghiyan, A.A. Vardanyan, and A.S. Saghiyan

Страница: 62-69

Isolation and Purification of Bioinhibitory Product from Culture Liquid of Lacticacid Bacteria Lactobacillus acidophilus 1991 VKPM 6257 and Lactobacillus rhamnosus INMIA 9614

Biotekhnologiya, 2015, N 4, P. 62-69

UDC 57.042.2:57.083.38

Section:  “Biologicals Technology”

A.E. Aghajanyan ^*,  F.N. Tkhruni,  G.Zh. Hovhannisyan,  K.I. Yeghiyan,  A.A. Vardanyan,  and  A.S. Saghiyan

Armbiotechnology, Natl. Acad. Sci. Republic of Armenia,  0056, Yerevan Armenia

e-mail:  aghajanyanarmen@yahoo.com

The process of the ion-exchange method for the isolation and purification of bioinhibitory product from culture liquids (CL) of lacticacid bacteria, Lactobacillus acidophilus and L. rhamnosus, has been studied and the optimum parameters of the process have been determined. To purify the target product from related admixtures the eluates after the ion-exchange isolation were subjected to extraction. The experiments showed that dimethylkretone and diethylkretone proved to be the most efficient extragents. The yield of the target product from the supernatant was ~ 64 % taking into account the solution return to the technological cycle. The bioinhibiting activity of the obtained product was equal to 28,000 AU/ml. The accompanying bactericidal effect of lactic acid on the growth of Gram-positive and Gram-negative cultures was only visible at pH ≤ 3.4 and concentration more than 0.5%.

Key words:  bioinhibitor,  extraction,  ion exchange,  isolation,  lactic acid.

06.11.2015, 1543 просмотра.