Obtaining of Biologically Active Human Interferon-α-2b from Nicotiana benthamiana Plants

Автор: E.N. Kosobokova, M.V. Piniugina, and V.S. Kosorukov

Страница: 52-61

Obtaining of Biologically Active Human Interferon-α-2b from Nicotiana benthamiana Plants

Biotekhnologiya, 2015, N 4, P. 52-61

UDC 57.088.2

Section:  “Producers, Biology, Selection, and Gene Engineering”

E.N. Kosobokova ^*,  M.V. Piniugina,  and  V.S. Kosorukov

The Blokhin Russian Oncologic Research Center,  115478, Moscow Russia

e-mail:  ekkos@mail.ru

A method for the production and purification of biologically active recombinant human interferon-α-2b (rhIFN-α-2b) that was synthesized as a result of expression in Nicotiana benthamiana plants has been developed. A genetic construct containing the modified gene of hIFN-α-2b was cloned in either of two vectors, first based on tobacco mosaic virus driven by the actin promoter of Arabidopsis thaliana (pA-IFN-A), and second, on cauliflower mosaic virus driven by the 35S promoter (pA-IFN-S). The expression vectors were delivered into the plant cells by agroinjection. The maximal synthesis rate was detected 5 days after the injection and was up to 200 mg and 20 mg per kg of fresh leaves for pA-IFN-A and pA-IFN-S, respectively. The recombinant plant-made hIFN-α-2b had as high antiviral and antitumor activity as a commercial drug.

Key words:  His-tag,  production in plants,  rhIFN-α-2b.

06.11.2015, 1283 просмотра.