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Biofilms of Nitrile-Hydrolyzing Bacteria: Dynamics of Growth, Resistance to Toxic Substances and Biotechnological Potential
Biofilms of Nitrile-Hydrolyzing Bacteria: Dynamics of Growth, Resistance to Toxic Substances and Biotechnological PotentialАвтор: Yu.G. Maksimova, A.Yu. Maksimov, and V.A. Demakov Страница: 39-51
Biofilms of Nitrile-Hydrolyzing Bacteria: Dynamics of Growth, Resistance to Toxic Substances and Biotechnological Potential Biotekhnologiya, 2015, N 4, P. 39-51 UDC 579.222 : 579.66 Section: “Producers, Biology, Selection, and Gene Engineering”
Yu.G. Maksimova 1, 2, *, A.Yu. Maksimov 1, 2, and V.A. Demakov 1, 2 1 The Institute for Ecology and Genetics of Microorganisms, Russ. Acad., Sci., Ural branch, 614081, Perm' Russia 2 The Perm' State National Research University, 614990, Perm' Russia e-mail: maks@iegm.ru
Mono- and mixed bacterial biofilms have been obtained during the Rhodococcus ruber gt1, Pseudomonas fluorescens C2, Alcaligenes faecalis 2, and Rh. erythropolis 11-2 growth in the presence of carriers. The transformation of aliphatic and aromatic nitriles by the biofilm of nitrile-hydrolyzing bacteria, as well as the growth dynamics of Rh. ruber gt1 and P. fluorescens C2 biofilms and their resistance to toxic substrates and products of the nitrile hydrolysis were studied. It was shown that P. fluorescens C2 biofilm mass and total ATP content reached a peak after a day of growth, whereas the same for Rh. ruber gt1 was observed after 3—4 days of culturing. The biofilms of Rh. ruber gt1 and P. fluorescens C2 were shown to be more resistant to the effects of high concentrations of acrylamide and acrylonitrile and to have greater adaptive capacity than planktonic cells.
Key words: amidase, biofilm, nitrilase, nitrile-hydrolyzing bacteria, nitrile hydratase.
06.11.2015, 1510 просмотров. |
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