Immunochromatographic Serodiagnosis of Tuberculosis using Colloidal Gold—Antigen Conjugate

Автор: D.V. Sotnikov, A.V. Zherdev, V.G. Avdienko, and B.B. Dzantiev

Страница: 76-81

Immunochromatographic Serodiagnosis of Tuberculosis using Colloidal Gold—Antigen Conjugate

Biotekhnologiya, 2015, N 2, P. 76-81

UDC 57.083.3: 616-002.5

Section:  “Metrology, Standartization, and Control”

D.V. Sotnikov ^{1, *},  A.V. Zherdev ¹,  V.G. Avdienko ²,  and  B.B. Dzantiev ^{1, *}

¹  The Bach Institute of Biochemistry, Federal Research Centre Fundamentals of Biotechnology, Russ. Acad. Sci.,  119071, Moscow Russia

²  The Central Tuberculosis Research Institute, Russ. Acad. Med. Sci.,  107564, Moscow Russia

e-mail:  dzantiev@inbi.ras.ru,  sotnikov-d-i-@mail.ru

A new technique for immunochromatographic serodiagnosis of tuberculosis that is based on the interaction of specific anti-mycobacterial immunoglobulins from blood serum with label—antigen conjugate and subsequent binding of the formed complex with the Staphylococcus aureus protein A immobilized on a test-strip has been suggested. Unlike the conventional technique in which all the immunoglobulins in a sample react with the label—immunoglobulin-binding protein conjugate, and then the labeled specific antibodies interact with an immobilized antigen, this technique prevents binding of the label to immunoglobulins that are not specific to Mycobacterium tuberculosis. The suggested assay was implemented using the recombinant 38 kDa (Rv0934) protein of M. tuberculosis as an antigen and colloidal gold as a label. It was experimentally shown that the proposed technique allows to increase the percentage of detection for seropositive serum with low concentrations of the specific antibodies to the M. tuberculosis cause.

Key words:  colloidal gold,  immunochromatography,  serodiagnosis,  tuberculosis.

25.06.2015, 1624 просмотра.