Obtaining of Components of Immunochromatography Test for Glanders and Melioidosis Causes Detection

Автор: G.N. Fediukina, S.S. Vetchinin, E.V. Baranova, S.Yu. Rudnitskii, P.V. Soloviov, N.V. Kolosova, and S.F. Biketov

Страница: 85-92

Obtaining of Components of Immunochromatography Test for Glanders and Melioidosis Causes Detection

Biotekhnologiya, 2015, N 1, P. 85-92

UDC 616.9:579.61

Section:  “Metrology, Standartization, and Control”

G.N. Fediukina ^*,  S.S. Vetchinin ^*,  E.V. Baranova,  S.Yu. Rudnitskii,  P.V. Soloviov,  N.V. Kolosova,  and  S.F. Biketov

The State Research Center for Applied Microbiology and Biotechnology,  142279, Obolensk, Moskovskaya oblast Russia

e-mail:  galafed@mail.ru,  vetchinin@obolensk.com

Hybridomas producing monoclonal antibodies to the lipopolysaccharide from Burkholderia mallei and Burkholderia pseudomallei have been obtained. Optimal conditions for the antibody conjugation with colloidal gold particles (20 nm) were selected. The dot-immunoanalysis showed that the obtained conjugates interact with the glanders and melioidosis causes and fail to cross-react with 3 Gram-positive and 5 Gram-negative bacteria strains. The obtained gold-conjugates were shown to be helpful in immunochromatography tests for the detection of the glanders and melioidosis causes.

Key words:  colloidal gold,  conjugate,  glanders,  immunochromatography,  melioidosis,  monoclonal antibodies.

29.05.2015, 1663 просмотра.