Investigation of Antimicrobial Properties of Silver Nanoparticles Stabilized by Maleic Acid Copolymers

Автор: N.A. Samoilova, M.A. Krayukhina, D.A. Popov, N.M. Anuchina, and I.A. Yamskov

Страница: 75-84

Investigation of Antimicrobial Properties of Silver Nanoparticles Stabilized by Maleic Acid Copolymers

Biotekhnologiya, 2015, N 1, P. 75-84

UDC 546.57:544.77.032.1:615.28

Section:  “Utilization of Biopreparations”

N.A. Samoilova ^{1, *},  M.A. Krayukhina ¹,  D.A. Popov ²,  N.M. Anuchina ²,  and  I.A. Yamskov ¹

¹  The Nesmeyanov Institute of Organoelement Compounds, Russ. Acad. Sci.,  119991, Moscow Russia

²  The Bakoulev Scientific Center for Cardiovascular Surgery,  121552, Moscow Russia

e-mail:  samoilova.nadezhda@gmail.com

Silver nanoparticles have been prepared by the reduction of the corresponding metal salt with NaBH₄ in the presence of appropriate dispersing agents, namely, copolymers of maleic acid with N-vinylpyrrolidone, ethylene and styrene or their amphyphilic derivative containing  octadecylamide ligands. The silver colloids identified by optical absorption spectroscopy were synthesized when the copolymer concentrations were lower than their critical aggregation concentrations and copolymer/Ag+ molar ratio no greater than a unit. According to the data of transmission electron microscopy, the obtained Ag nanoparticles were spherical-shaped particles 1—2 nm in size. Colloidal solutions of the prepared nanosilver showed a strong inhibiting antifungal activity against a fungal plant pathogen Fusarium oxysporum and also Gram-negative, Gram-positive bacteria and yeast. The silver nanoparticles stabilized with more hydrophilic compounds from among the investigated copolymers demonstrated the higher antibacterial and antifungal activity.

Key words:  antibacterial activity,  antifungal activity,  copolymers of maleic acid,  silver nanoparticles.

29.05.2015, 1605 просмотров.