Efficiency of CHO Сell Culture Supplementation with Fatty Acids, N-acetyl-D-mannosamine and N-acetylneuraminic Acid for Modification of Recombinant Darbepoetin alpha Sialylation

Автор: I.N. Savinova, N.V. Lobanova, N.N. Bykova, Yu.V. Finogeeva, L.I. Starodubtseva, A.A. Klishin, A.A. Nurbakov, R.R. Shukurov, and Yu.A. Seryogin

Страница: 22-28

Efficiency of CHO Сell Culture Supplementation with Fatty Acids, N-acetyl-D-mannosamine and N-acetylneuraminic Acid for Modification of Recombinant Darbepoetin alpha Sialylation

Biotekhnologiya, 2015, N 1, P. 22-28

UDC 602.6:612

Section:  “Biologicals Technology”

I.N. Savinova ¹,  N.V. Lobanova ^{1, *},  N.N. Bykova ¹,  Yu.V. Finogeeva ¹,  L.I. Starodubtseva ¹,  A.A. Klishin ¹,  A.A. Nurbakov ¹,  R.R. Shukurov ¹,  and  Yu.A. Seryogin ²

¹  The Limited Liability Company Pharmapark,  117246, Moscow Russia

²  The State Research Institute for Genetics and Selection of Industrial Microorganisms,  117545, Moscow Russia

e-mail:  natalya.lobanova@pharmapark.ru

The selection of medium—feed combination for the improvement of the target glycoforms yield of recombinant darbepoetin alpha produced by CHO cell culture has been performed. The impact of various glycan precursors in the glycosylation process on the yield of highly sialylated darbepoein alpha forms was investigated. The supplementation of the cell culture with 20 mM N-acetyl-D-mannosamine was shown to be most effective for the increase in highly sialylated darbepoein alpha forms content.

Key words:  CHO cells,  darbepoetin alpha,  fatty acids,  glycosylation,  sialylation.

29.05.2015, 1776 просмотров.