Fermentation Optimization of a Saccharomyces cerevisiae Strain Producing 1F9 Recombinant Spidroin

Автор: K.V. Sidoruk, L.I. Davydova, D.G. Kozlov, I.I. Gubaidullin, A.V. Glazunov, V.G. Bogush, and V.G. Debabov

Страница: 27-35

Fermentation Optimization of a Saccharomyces cerevisiae Strain Producing 1F9 Recombinant Spidroin

Russian Journal of Biotechnology, 2014, N 6, P. 27-35

UDC 577.112.083

Section:  “Biologicals Technology”

K.V. Sidoruk ^*,  L.I. Davydova,  D.G. Kozlov,  I.I. Gubaidullin,  A.V. Glazunov,  V.G. Bogush,  and  V.G. Debabov

The State Research Institute for Genetics and Selection of Industrial Microorganisms,  117545, Moscow Russia

e-mail:  k_sidoruk@mail.ru

The optimization of the fermentation of a Saccharomyces cerevisiae strain producing recombinant spidroin 1F9 has been carried out. A simplified two-stage scheme of the process was developed; the effect of sucrose, glucose, fructose and galactose on the efficiency of the process was investigated. The optimum concentration of sucrose in the medium  and recharge was determined. The influence of peptone, tryptone and casein hydrolysate of various brands on the effectiveness of the fermentation was analyzed. The optimum concentrations of peptone and yeast extract in the medium and recharge were determined. As a result, owing to the optimization, the process of fermentation was simplified, a new composition of a complex feeding was designed and sucrose specific consumption was reduced by 2 times, whereas the expenses of peptone and yeast extract were decreased by about 2.5 times, the yield of biomass per unit of the culture broth volume grew by ~ 40%, and that of the protein of interest increased by ~ 60%.

Key words:  fermentation,  galactose,  peptone,  recharge,  recombinant spidroin,  saccharomyces yeast,  strain-producer,  sucrose,  yeast extract.

31.03.2015, 1585 просмотров.