Microbial Components of Kefir Grains as Producers of Exopolysaccharide Kefiran

Автор: N.B. Gradova, A.A. Khokhlacheva, E.D. Murzina, and V.V. Myasoyedova

Страница: 18-26

Microbial Components of Kefir Grains as Producers of Exopolysaccharide Kefiran

Russian Journal of Biotechnology, 2014, N 6, P. 18-26

UDC 579.674; 663.15

Section:  “Producers, Biology, Selection, and Gene Engineering”

N.B. Gradova ^{1, *},  A.A. Khokhlacheva ^{1, *},  E.D. Murzina ¹,  and  V.V. Myasoyedova ²

¹  The Mendeleyev Russian University of Chemical Technology,  125047, Moscow Russia

²  The Semenov Institute of Chemical Physics, Russ. Acad. Sci.,  119991, Moscow Russia

e-mail:  gradova_nb@mail.ru,  sasha-88888@yandex.ru

A screening of lactic acid polysaccharide-synthesizing bacteria, components of the kefir grains, has shown that about 60% of isolates are able to synthesize exopolysaccharides (EPS). The most active cultures, Lactococcus lactis and Leuconostoc mesenteroides, that synthesize exopolysaccharides during the development on a medium with lactose or sucrose, respectively, were selected. The results of IR spectroscopy confirmed the identity of the compositions of the synthesized exopolysaccharides with polysaccharides of kefir grains. A culturing regime for L. mesenteroides on whey supplemented with sucrose that provides the production level of kefiran up to 3,4 g/l was developed.

Key words:  kefiran,  kefir grains,  lactic acid bacteria,  synthesis and composition of exopolysaccharides.

31.03.2015, 1707 просмотров.