Functional Analysis of Rpn4-like Proteins from Komagataella (Pichia) pastoris and Yarrowia lipolytica in Heterological system of Saccharomyces cerevisiae

Автор: E.N. Grineva, A.T. Leinsoo, D.S. Spasskaya, D.S. Karpov, and V.L. Karpov

Страница: 8-17

Functional Analysis of Rpn4-like Proteins from Komagataella (Pichia) pastoris and Yarrowia lipolytica in Heterological system of Saccharomyces cerevisiae

Russian Journal of Biotechnology, 2014, N 6, P. 8-17

UDC 577.218

Section:  “Producers, Biology, Selection, and Gene Engineering”

E.N. Grineva ¹,  A.T. Leinsoo ¹,  D.S. Spasskaya ^{1, 2},  D.S. Karpov ^{1, 3, *},  and  V.L. Karpov ¹

¹  The Engelhardt Institute for Molecular Biology,  119991, Moscow Russia

²  The State Research Institute for Genetics and Selection of Industrial Microorganisms,  117545, Moscow Russia

³  The Orekhovich Research Institute for Biomedical Chemistry,  119121, Moscow Russia

e-mail:  aleom@yandex.ru

The expression of Saccharomyces cerevisiae proteasomal genes is regulated coordinately by a system consisting of the ScRpn4 transcription factor and its binding site called PACE. The analysis of genomes permitted to establish that only yeasts of the Saccharomycetes class have genes for ScRpn4 orthologs and PACE-like elements in the promoters of proteasomal genes. This taxonomic group contains such species of biotechnological importance as Komagataella (Pichia) pastoris and Yarrowia lipolytica. To date, ScRpn4 orthologs from these yeasts have not been characterized experimentally. In the present work, using the heterological expression system of S. cerevisiae, we showed that ScRpn4 orthologs from K. pastoris and Y. lipolytica restore the mRNA levels of proteasomal genes and stress resistance in the mutant strain with a deletion of the SCRPN4 gene, i.e., they are similar in function with ScRpn4 from S. cerevisiae.

Key words:  Komagataella (Pichia) pastoris,  proteasomal genes,  Rpn4,  transcription regulation,  Yarrowia lipolytica.

31.03.2015, 1830 просмотров.