Obtaining and Study of Complexes of the Polypyrrol—Polyene Antibiotic Type

Автор: A.V. Timofeeva, N.М. Maliutina, E.A. Stepashkina, L.A. Baratova, I.Yu. Sapurina, and G.S. Katrukha

Страница: 50-58

Obtaining and Study of Complexes of the Polypyrrol—Polyene Antibiotic Type

Russian Journal of Biotechnology, 2014, N 5, P. 50-58

UDC 577.181.3

Section:  “Biologicals Technology”

A.V. Timofeeva ^{1, *},  N.М. Maliutina ²,  E.A. Stepashkina ²,  L.A. Baratova ¹,  I.Yu. Sapurina ³,  and  G.S. Katrukha ²

¹  The Belozersky Research Institute for Physicochemical Biology, Lomonosov Moscow State University,  119991, Moscow Russia

²  The Gause Institute of New Antibiotics, Russ. Acad. Med. Sci.,  119021, Moscow Russia

³  The Institute of Macromolecular Compounds, Russ. Acad. Sci.,  199004, St.-Petersburg Russia

e-mail:  timofeeva@belozersky.msu.ru

Complexes of practically important polyene antibiotics (amphotericin B and nystatin A₁) with a modern biocompatible electroactive polymer polypyrrol (PP) were obtained for the first time, and their antifungal activities and some physicochemical properties were studied. It was established that the PP—amphotericin B complex possesses the antifungal activity against Aspergillus niger, whereas the PP—nystatin A₁ complex is devoid of this activity. It was shown by infra-red spectroscopy that the basis for the adsorption of amphotericin B on PP is the formation of an ionic bond between the COOH-group of the polyene antibiotic and the NH-group of the pyrrol residue, and also the interaction of the polyene system of the antibiotic with the PP polymer chain. The inactivation of the PP—nystatin A₁ complex is supposed to be connected with the complete shielding during the complex formation of the polyene fragment of the antibiotic, and also of amine and carboxylic groups in nystatin A₁ that are responsible for the primary binding of the antibiotic to the micromycete membrane.

Key words:  antifungal activity,  antimycotics,  Aspergillus niger,  complexes of the polypyrrol—antibiotic type,  infra-red spectroscopy,  micromycete,  polyene antibiotics,  polypyrrol.

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