Russian Journal of Biotechnology Articles archive Archive 2014 N 5 Method for Producing of Virus-Like Particles from Saccharomyces cerevisiaе Killer Yeast Strain and their Properties

Method for Producing of Virus-Like Particles from Saccharomyces cerevisiaе Killer Yeast Strain and their Properties

Автор: Z.A. Shevchenko, L.R. Lebedev, V.P. Klimenko, V.I. Masycheva, S.G. Gamaley, A.V. Bateneva, E.D. Danilenko, E.A. Ilyasov, M.Sh. Azaev, and B.N. Zaitsev

Страница: 32-37

 

Method for Producing of Virus-Like Particles from Saccharomyces cerevisiaе Killer Yeast Strain and their Properties

Russian Journal of Biotechnology, 2014, N 5, P. 32-37

UDC 578.89:615.281+612.017

Section:  “Producers, Biology, Selection, and Gene Engineering”

 

Z.A. Shevchenko,  L.R. Lebedev *,  V.P. Klimenko,  V.I. Masycheva,  S.G. Gamaley,  A.V. Bateneva,  E.D. Danilenko,  E.A. Ilyasov,  M.Sh. Azaev,  and  B.N. Zaitsev

The State Research Center of Virology and Biotechnology «Vector»,  633010, Berdsk, Novosibirskaya Oblast Russia

e-mail:  lebedev_lr@vector.nsc.ru

 

On the basis of the developed technology for obtaining of virus-like particles from the killer yeast strain of Saccharomyces cerevisiae Y-448, including stages of extraction, concentration and chromatographic purification, the experimental series of preparations were produced and their properties were investigated. Particle sizes were determined by the electron microscopy method. It was established that dsRNAs within the particle structure are characterized by a higher enzyme resistance as compared to free dsRNA. In in vivo experiments, it was shown that the VLP preparations enhance the phagocytic activity of macrophages and interferon level in mouse serum, and this effect was higher in VLP than in the preparation of comparison (Ridostin).

 

Key words:  capsid protein,  double-stranded RNA,  interferon induction,  phagocytosis,  RNAse,  virus-like particle.

 

24.03.2015, 1592 просмотра.

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